Active Recombinant Human BTN3A2 protein, His/Avi-tagged, Biotinylated
Cat.No. : | BTN3A2-051H |
Product Overview : | Biotinylated Recombinant Human BTN3A2(Gln30-Trp248) protein, fused to His/Avi tag at the C-terminus, was expressed in HEK293 cells . |
- Specification
- Gene Information
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Description : | BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region with one V-type Ig-like domain and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. A rodent counterpart to BTN3A2 has not been reported. BTN3A2 mRNA over‑expression was associated with a good prognosis in relation to disease-free and overall survival in a cohort of 55 epithelial ovarian cancer (EOC) patients (1). Another study in a larger cohort of 199 high-grade EOC patients further confirmed that the protein expression of BTN3A2 in ovarian cancer tissues is positively correlated with the intraepithelial infiltration of CD4+ and CD8+ T cells (2), suggesting that BTN3A2 was a co-stimulatory molecule to modulate the infiltration of immune cells and thus the anti-cancer immunity. In consistent with previous publications, our in-house studies on BTN3A2 showed that BTN3A2 co-stimulated anti‑CD3 induced IFN-gamma secretion on CD3+ cells. |
Source : | HEK293 cells |
Species : | Human |
Tag : | His/Avi |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Sheep Anti-Human BTN3A1/2 Antigen Affinity-purified Polyclonal Antibody is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human BTN3A2 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 15-75 ng/mL. |
Molecular Mass : | 27-37 kDa, under reducing conditions |
Protein length : | Gln30-Trp248 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 200 μg/mL in PBS. |
Gene Name : | BTN3A2 butyrophilin, subfamily 3, member A2 [ Homo sapiens ] |
Official Symbol : | BTN3A2 |
Synonyms : | BTN3A2; butyrophilin, subfamily 3, member A2; butyrophilin subfamily 3 member A2; butyrophilin protein; BTF4; BT3.2; BT3.3; FLJ40011; |
Gene ID : | 11118 |
mRNA Refseq : | NM_001197246 |
Protein Refseq : | NP_001184175 |
MIM : | 613594 |
UniProt ID : | P78410 |
Products Types
◆ Recombinant Protein | ||
BTN3A2-1739H | Recombinant Human BTN3A2 Protein (30-248 aa), His-tagged | +Inquiry |
BTN3A2-458H | Recombinant Human BTN3A2 Protein, His-Avi-tagged, Biotinylated | +Inquiry |
BTN3A2-460H | Recombinant Human BTN3A2 Protein, His (Fc)-Avi-tagged | +Inquiry |
BTN3A2-364H | Recombinant Human BTN3A2 Protein (30-248 aa), His-SUMO-tagged | +Inquiry |
BTN3A2-0796H | Recombinant Human BTN3A2 Protein (Gln30-Trp248), C-His tagged | +Inquiry |
◆ Lysates | ||
BTN3A2-8385HCL | Recombinant Human BTN3A2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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