Active Recombinant Human CCL1 Protein (74 aa)

• Cat.No.: CCL1-383C
• Product Overview: Recombinant Human I-309/CCL1 produced in E. coli is a single non-glycosylated polypeptide chain containing 74 amino acids. A fully biologically active molecule, rhI-309/CCL1has a molecular mass of 8.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.

Specification

• Description: Chemokine (C-C motif) ligand 1 (CCL1), also known as I-309, is a small glycoprotein secreted by activated T cells that belongs to the family ofchemokines. Human CCL1 has been assumed to be a homologue of mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. CCL1 attracts monocytes, NK cells, immature B cells and dendritic cells by interacting with the cell surface chemokine receptor CCR8. This chemokine resides in a large cluster of CC chemokines on human chromosome 17.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The EC50 value of human I-309/CCL on Ca^2+ mobilization assay in CHO-K1/Gα15/hCCR8 cells (human Gα15 and human CCR8 stably expressed in CHO-K1 cells) is less than 1 μg/mL.
• Molecular Mass: 8.5 kDa, observed by reducing SDS-PAGE.
• Protein length: 74
• AA Sequence: SKSMQVPFSRCCFSFAEQEIPLRAI LCYRNTSSICSNEGLIFKLKRGKEA CALDTVGWVQRHRKMLRHCPSKRK
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant human I-309/CCL1 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, recombinant human I-309/CCL1 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: CCL1 chemokine (C-C motif) ligand 1 [ Homo sapiens ]
• Official Symbol: CCL1
• Synonyms: CCL1; chemokine (C-C motif) ligand 1; SCYA1, small inducible cytokine A1 (I 309, homologous to mouse Tca 3); C-C motif chemokine 1; I 309; inflammatory cytokine I 309; P500; SISe; T lymphocyte secreted protein I 309; TCA3; inflammatory cytokine I-309; small-inducible cytokine A1; T lymphocyte-secreted protein I-309; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); I-309; SCYA1
• Gene ID: 6346
• mRNA Refseq: NM_002981
• Protein Refseq: NP_002972
• MIM: 182281
• UniProt ID: P22362

Reviews

11/14/2022

Its exceptional purity and integrity ensure clear and reliable bands, enabling accurate detection and quantification of target proteins.

12/03/2019

Its stability and structural integrity make it an ideal candidate for studying protein structures at high resolution.

11/11/2019

I am delighted to highly recommend the aroE Protein for researchers seeking excellent performance in Western blotting (WB) experiments and protein electron microscopy structure analysis

Q&As

How does CCL1 contribute to the pathogenesis of inflammatory conditions?

CCL1 promotes the recruitment of immune cells to inflamed tissues, exacerbating inflammation and contributing to the pathogenesis of various inflammatory conditions.

Are there any ongoing clinical trials targeting CCL1 for therapeutic intervention?

Several clinical trials are exploring the therapeutic potential of targeting CCL1 in autoimmune diseases and inflammatory disorders to modulate immune responses.

How does CCL1 interact with other immune modulators, and what implications does this have for drug development?

CCL1 interacts with various immune modulators, and understanding these interactions is crucial for developing targeted therapies that can modulate the immune response more effectively.

In what ways does CCL1 participate in the regulation of immune responses?

CCL1 regulates immune responses by influencing the migration and activation of specific immune cells, modulating the overall immune system's activity.

How does CCL1 influence the migration of specific immune cell types, and how is this mechanism exploited in clinical applications?

CCL1 selectively influences the migration of specific immune cell types, and understanding this mechanism is essential for developing therapies that target specific immune responses in various diseases.