Recombinant Human CCL1, His-tagged

• Cat.No.: CCL1-168H
• Product Overview: Recombinant human CCL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CCL1 (chemokine C-C motif ligand 1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Unlike the other members of the C-C family, CCL1 does not induce chemotaxis in natural killer (NK) cells.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 10% glycerol, 50mM NaCl.
• Molecular Weight: 10.8 kDa (94aa) confirmed by MALDI-TOF.
• Purity: > 85% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Cytokine-cytokine receptor interaction

Gene Information

• Gene Name: CCL1 chemokine (C-C motif) ligand 1
• Synonyms: P500; SISe; TCA3; I-309; SCYA1; small inducible cytokine A1; inflammatory cytokine I-309; T lymphocyte-secreted protein I-309; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); chemokine (C-C motif) ligand 1; CCL1
• Gene ID: 6346
• mRNA Refseq: NM_002981
• Protein Refseq: NP_002972
• MIM: 182281
• UniProt ID: P22362
• Chromosome Location: 17q12
• Function: chemokine activity