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Active Recombinant Human CD47 protein, His-tagged

Cat.No. : CD47-2186H
Product Overview : Recombinant Human CD47 protein(NP_942088.1)(Met1-Pro139), fused with His tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS,pH7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha hFc at 2 μg/ml (100 μl/well) can bind Human CD47 His, the EC50 of Human CD47 His is 5.0-25.0 ng/mL.
Molecular Mass : The recombinant human CD47 consists of 132 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 35-44 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein Length : Met1-Pro139
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : CD47 CD47 molecule [ Homo sapiens ]
Official Symbol : CD47
Synonyms : CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer) , MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6;
Gene ID : 961
mRNA Refseq : NM_001777
Protein Refseq : NP_001768
MIM : 601028
UniProt ID : Q08722

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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