Recombinant Cynomolgus/Rhesus macaque CD47 protein, His-tagged

• Cat.No.: CD47-346C
• Product Overview: Recombinant Cynomolgus/Rhesus macaque CD47 protein (Gln19-Glu141), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).In the region Gln19-Glu141, the AA sequence of Cynomolgus and Rhesus macaque CD47 are homologus.

Specification

• Description: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
• Source: HEK293
• Species: Cynomolgus/Rhesus macaque
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 15.8 kDa. The protein migrates as 30-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 123
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD47
• Official Symbol: CD47
• Synonyms: IAP; MER6; OA3
• Gene ID: 704980
• mRNA Refseq: NM_001266517.1
• Protein Refseq: NP_001253446.1
• UniProt ID: F7A802