Active Recombinant Human CES1 Protein (His19-Glu563), C-10×His tagged

• Cat.No.: CES1-13H
• Product Overview: Recombinant Human Carboxylesterase 1/CES1 Protein (His19-Glu563) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is > 6000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 61 kDa
  SDS-PAGE: 61 kDa, reducing conditions
• Protein length: His19-Glu563
• N-terminal Sequence Analysis: His19
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: CES1 carboxylesterase 1 [ Homo sapiens (human) ]
• Official Symbol: CES1
• Synonyms: CES1; carboxylesterase 1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1; liver carboxylesterase 1; acyl coenzyme A:cholesterol acyltransferase; brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); carboxylesterase 2 (liver); cholesteryl ester hydrolase; cocaine carboxylesterase; egasyn; human monocyte/macrophage serine esterase 1; methylumbelliferyl-acetate deacetylase 1; monocyte/macrophage serine esterase; retinyl ester hydrolase; serine esterase 1; triacylglycerol hydrolase; EC 3.1.1.1; EC 3.1.1.13; EC 3.1.1.56
• Gene ID: 1066
• mRNA Refseq: NM_001025194
• Protein Refseq: NP_001020365
• MIM: 114835
• UniProt ID: P23141

Reviews

03/29/2022

Reliable for lab work.

09/16/2021

Great value for quality.

09/03/2020

Good quality, great service.

Q&As

What is the primary function of the CES1 enzyme in the human body?

CES1 hydrolyzes ester and amide bonds in various compounds, including drugs, xenobiotics, and endogenous molecules.

In which tissues or organs is CES1 most abundantly expressed?

CES1 is most abundantly expressed in the liver, playing a significant role in first-pass metabolism.

How does CES1 contribute to drug metabolism?

It metabolizes numerous ester-containing drugs, affecting their pharmacokinetics and pharmacodynamics.

What is the significance of CES1 in the metabolism of prodrugs?

CES1 is crucial for the activation of certain prodrugs, converting them into their active pharmacological forms.

How can genetic variations in the CES1 gene affect drug response?

Genetic variations in CES1 can lead to inter-individual differences in drug metabolism, impacting efficacy and toxicity.

Are there known inhibitors or activators of CES1, and how might they influence its activity?

Certain compounds can inhibit or activate CES1, altering the metabolism of its substrates.

What role does CES1 play in lipid metabolism?

It participates in the hydrolysis of triglycerides and cholesterol esters, contributing to lipid homeostasis.