Active Recombinant Human Chemokine (C-C Motif) Ligand 19

• Cat.No.: CCL19-298H
• Product Overview: Recombinant Human MIP-3b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8809 Dalton.

Specification

• Cat. No.: CCL19-298H
• Description: This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes.
• Species: Human
• Source: E.coli
• Form: Lyopohilized with no additives.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: Macrophage inflammatory protein 3beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human T cells using a concentration of 7-40 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: CCL19 chemokine (C-C motif) ligand 19 [ Homo sapiens ]
• Official Symbol: CCL19
• Synonyms: small inducible cytokine A19; exodus-3; CK beta-11; EBI1-ligand chemokine; CC chemokine ligand 19; beta chemokine exodus-3; small inducible cytokine subfamily A (Cys-Cys), member 19; ELC; CKb11; MIP3B; MIP-3b; SCYA19; MGC34433; CCL19; Macrophage inflammatory protein 3 beta; Short name=MIP-3-beta; EBI1-ligand chemokine; Short name=ELC; Beta chemokine exodus-3; CK beta-11; C-C motif chemokine 19; ELC; MIP3B; SCYA19
• Gene ID: 6363
• mRNA Refseq: NM_006274
• Protein Refseq: NP_006265
• MIM: 602227
• UniProt ID: Q99731
• Chromosome Location: 9p13
• Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Signaling by GPCR
• Function: chemokine activity