Active Recombinant Human CUL1/RBX1/SKP1

• Cat.No.: CUL1-147H
• Product Overview: Recombinant human CUL1 (amino acid residues 1-776), RBX1 (amino acid residues 1-108), SKP1 (amino acid residues 1-160) was expressed in Insect (Sf21) cells.

Specification

• Description: Cullin-RING-Ligases (CRLs) are one largest class of ubiquitin E3 ligases and the enzymes of the NEDDylation pathway play a pivotal role in the activation of these, akin to ubiquitylation, the E1 activating enzyme (APP-BP1/UBA3 heterodimer) and the E2 conjugating enzymes (UBE2M or UBE2F) are involved in mammalian NEDDylation of the Cullin Ring Ligases (CRLs) (Meyer-Schaller et al., 2009; Huang et al., 2011; Morimoto et al., 2003).
• Source: Insect cells
• Species: Human
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: E3 Ligase Assay: The activity of Cul1/Rbx1/Skp1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase GST-DCNL1 and thioester-loaded His-Ube2M-NEDD8. Incubation of Cul1/Rbx1/Skp1 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of GST-DCNL1 at 4?C was compared at two time points T0 and T2 minutes. Increased Neddylation of the Cul1 subunit in the presence of GST-DCNL1 was demonstrated.
• Molecular Mass: Cul1: ~89.9 kDa; Rbx1: ~12.3 kDa; Skp1: ~18.3
• Purity: >95% by InstantBlue™ SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml
• Publication:
  Lys29-linkage of ASK1 by Skp1? Cullin 1? Fbxo21 ubiquitin ligase complex is required for antiviral innate response." (2016)

Gene Information

• Gene Name: CUL1 cullin 1 [ Homo sapiens ]
• Official Symbol: CUL1
• Synonyms: CUL1; cullin 1; cullin-1; CUL-1; MGC149834; MGC149835
• Gene ID: 8454
• mRNA Refseq: NM_003592
• Protein Refseq: NP_003583
• MIM: 603134
• UniProt ID: Q13616
• Chromosome Location: 7q36.1
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Cell Cycle, organism-specific biosystem
• Function: protein binding; ubiquitin protein ligase binding