FGF18-522H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human FGF18 produced in E. coli is approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.
Description :
Human FGF-18 is encoded by the FGF18 gene. By phylogenetic analysis and gene location analysis, FGF-18 is divided into FGF-8 subfamily which has three members FGF-8, FGF-17 and FGF-18. Using FGF knockout mice model, the numbers of this subfamily were testified that have crucial roles of in embryo development. FGF-18–/– mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF-18 has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. In addition, it also has significant roles in lung development and has an anabolic effect on cartilage formation.
Source :
E. coli
Species :
Human
Form :
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity :
Fully biologically active when compared to standard. The ED50 determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0 × 10^6 IU/mg in the presence of 1μg/ml of heparin.
Less than 1EU/μg of rHuFGF-18 as determined by LAL method.
Purity :
>97% by SDS-PAGE and HPLC analyses.
Usage :
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage :
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution :
We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.