Active Recombinant Human FGF21 Protein (182 aa)
Cat.No. : | FGF21-120F |
Product Overview : | Recombinant Human FGF21 Protein without tag was expressed in E. coli. |
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Description : | Fibroblast growth factor 21 (FGF21) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors and stimulates insulin independent glucose uptake by adipocytes. |
Source : | E. coli |
Species : | Human |
Form : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of BaF3 cells expressing FGF receptors is 0.06-0.4 μg/mL in the presence of betaKlotho and Heparin, corresponding to a Specific Activity of >2.5× 10^3 IU/mg. |
Molecular Mass : | Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
Protein Length : | 182 |
AA Sequence : | MHPIPDSSPLLQFGGQVRQRYLYTD DAQQTEAHLEIREDGTVGGAADQSP ESLLQLKALKPGVIQILGVKTSRFL CQRPDGALYGSLHFDPEACSFRELL LEDGYNVYQSEAHGLPLHLPGNKSP HRDPAPRGPARFLPLPGLPPALPEP PGILAPQPPDVGSSDPLSMVGPSQG RSPSYAS |
Endotoxin : | Less than 1 EU/mg of rHuFGF-21 as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analyses. |
Storage : | This lyophilized preparation is stable for several weeks at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
Storage Buffer : | Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | FGF21 fibroblast growth factor 21 [ Homo sapiens ] |
Official Symbol : | FGF21 |
Synonyms : | FGF21; fibroblast growth factor 21; FGF-21; |
Gene ID : | 26291 |
mRNA Refseq : | NM_019113 |
Protein Refseq : | NP_061986 |
MIM : | 609436 |
UniProt ID : | Q9NSA1 |
Products Types
◆ Recombinant Protein | ||
FGF21-85H | Active Recombinant Human FGF21 Protein | +Inquiry |
FGF21-415F | Active Recombinant Human FGF21 Protein (182 aa) | +Inquiry |
Fgf21-1490R | Recombinant Rat Fgf21 Protein, His-tagged | +Inquiry |
FGF21-4111H | Recombinant Human FGF21 Protein, GST-tagged | +Inquiry |
FGF21-117H | Active Recombinant Human FGF21 Protein (His29-Ser209), C-His tagged, Animal-free, Carrier-free | +Inquiry |
◆ Lysates | ||
FGF21-1890MCL | Recombinant Mouse FGF21 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewThe manufacturer's history of producing high-quality products contributed to our trust in this protein.
Collaboration with other research groups was made easier by the product's standardized use in the scientific community.
The product's role in promoting collaboration and teamwork positively impacted the quality of our research.
Q&As (7)
Ask a questionFGF21 expression in adipose tissue is regulated by various factors, including peroxisome proliferator-activated receptor gamma (PPARγ) activation.
FGF21 influences lipid metabolism by promoting the utilization of fatty acids for energy, contributing to the regulation of lipid homeostasis.
FGF21 levels are being explored as potential biomarkers for metabolic health, reflecting conditions such as insulin resistance and metabolic syndrome.
Yes, FGF21 plays a role in improving insulin sensitivity, making it a potential target for interventions in insulin resistance-associated conditions.
Yes, FGF21 influences energy expenditure by promoting thermogenesis in brown adipose tissue, contributing to overall metabolic regulation.
Gender-specific differences in FGF21 levels have been reported, with some studies suggesting higher circulating FGF21 concentrations in females.
FGF21 activates signaling pathways such as the AMP-activated protein kinase (AMPK) pathway, which contributes to its metabolic effects.
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