Active Recombinant Human FGF8 Protein (Gln52-Arg223), C-His tagged, Animal-free, Carrier-free

• Cat.No.: FGF8-100H
• Product Overview: Recombinant Human FGF8 Protein (Gln52-Arg223) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Fibroblast Growth Factors-8b (FGF-8b) is a 21.3 kDa member of the fibroblast Growth Factors with 183 amino acid residues. FGF-8 is an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In embryogenesis function, FGF8 is necessary in limb, brain, eye and ear formation. FGF-8b having a more potent inductive activity due to higher affinity for Fgf receptors.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 1.4-3.8 ng/mL. The specific activity of recombinant human FGF-8b is > 2 × 10⁵ IU/mg.
• Molecular Mass: The protein has a calculated MW of 22.14 kDa.
  The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Gln52-Arg223
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: FGF8 fibroblast growth factor 8 (androgen-induced) [ Homo sapiens (human) ]
• Official Symbol: FGF8
• Synonyms: FGF8; fibroblast growth factor 8 (androgen-induced); fibroblast growth factor 8; AIGF; androgen-induced growth factor; heparin-binding growth factor 8; KAL6; FGF-8; HBGF-8; MGC149376
• Gene ID: 2253
• mRNA Refseq: NM_001206389
• Protein Refseq: NP_001193318
• MIM: 600483
• UniProt ID: P55075

Reviews

07/08/2021

Top-quality and timely delivery. It's a pleasure working with this product.

06/30/2020

Service, quality, and shipping—all exceptional. This product is our research ally.

10/09/2019

Seamless shipping and a product that never disappoints. Kudos!

Q&As

How does FGF8 participate in embryonic development, particularly in the patterning of the midbrain and limb buds, and what molecular events coordinate its spatial and temporal expression?

FGF8 is essential for midbrain and limb bud patterning during embryonic development. Spatial and temporal expression is coordinated by signaling pathways and transcription factors that regulate FGF8 expression.

How do post-translational modifications, such as glycosylation, impact the function of FGF8, and what is the significance of these modifications in modulating ligand-receptor interactions?

Post-translational modifications, including glycosylation, can influence FGF8 function. Glycosylation may modulate ligand-receptor interactions, affecting the specificity and strength of FGF8 signaling.

What is the structural classification of FGF8, and how does its tertiary structure influence interactions with FGF receptors and heparin sulfate proteoglycans?

FGF8 is a member of the fibroblast growth factor family with a β-trefoil tertiary structure. This structure dictates its binding to FGF receptors and heparin sulfate proteoglycans, essential for signal transduction.

How does FGF8 participate in the crosstalk between different signaling pathways, such as Wnt and Shh, and what are the consequences for tissue morphogenesis and organogenesis?

FGF8 engages in crosstalk with pathways like Wnt and Shh during tissue morphogenesis. This interaction influences organogenesis by coordinating cellular processes and ensuring proper tissue patterning.

Can you discuss the interactions between FGF8 and the extracellular matrix, and how these interactions influence the stability and bioavailability of FGF8 ligands in the tissue microenvironment?

FGF8 interacts with the extracellular matrix, affecting ligand stability and bioavailability. This interaction modulates FGF8 diffusion and gradients in the tissue microenvironment.