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Recombinant Mouse Fgf8 protein

Cat.No. : Fgf8-539M
Product Overview : Recombinant Mouse Fgf8 protein was expressed in Escherichia coli.
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  • Gene Information
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Description : Murine FGF-8 is a heparin binding growth factor belonging to the FGF family, which plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Murine FGF-8 is first purified from an androgen-dependent mouse mammary carcinoma cell line as an androgen induces secretion. Cloning and analysis of the murine FGF8 gene revealed at least eight potential protein isoforms (FGF-8a-h). Murine FGF-8a and b share 100 % amino acid identity with that in humans, and murine FGF-8e and f share 98 % amino acid identity with humans. None of the FGF-8 isoforms exhibited activity to FGFR1b, 2b, 3b, but FGFR2c, 3c and FGFR4 can be activated by several FGF-8 isoforms. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration, and it is required for normal brain, eye, ear and limb development during embryogenesis.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg in the presence of 10 μg/ml of heparin.
Molecular Mass : Approximately 28.1 kDa, a single non-glycosylated polypeptide chain containing 246 amino acids.
Protein length : 246
AA Sequence : QVRSAAQKRGPGAGNPADTLGQGHE DRPFGQRSRAGKNFTNPAPNYPEEG SKEQRDSVLPKVTQRHVREQSLVTD QLSRRLIRTYQLYSRTSGKHVQVLA NKRINAMAEDGDPFAKLIVETDTFG SRVRVRGAETGLYICMNKKGKLIAK SNGKGKDCVFTEIVLENNYTALQNA KYEGWYMAFTRKGRPRKGSKTRQHQ REVHFMKRLPRGHHTTEQSLRFEFL NYPPFTRSLRGSQRTWAPEPR
Endotoxin : Less than 1 EU/µg of rMuFGF-8 as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Fgf8
Official Symbol : Fgf8
Synonyms : FGF8; fibroblast growth factor 8; HBGF-8; androgen-induced growth factor; heparin-binding growth factor 8; Aigf; Fgf-8; MGC59627;
Gene ID : 14179
mRNA Refseq : NM_001166361
Protein Refseq : NP_001159833
UniProt ID : P37237

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does FGF8 participate in embryonic development, particularly in the patterning of the midbrain and limb buds, and what molecular events coordinate its spatial and temporal expression? 06/03/2021

FGF8 is essential for midbrain and limb bud patterning during embryonic development. Spatial and temporal expression is coordinated by signaling pathways and transcription factors that regulate FGF8 expression.

How do post-translational modifications, such as glycosylation, impact the function of FGF8, and what is the significance of these modifications in modulating ligand-receptor interactions? 09/14/2020

Post-translational modifications, including glycosylation, can influence FGF8 function. Glycosylation may modulate ligand-receptor interactions, affecting the specificity and strength of FGF8 signaling.

What is the structural classification of FGF8, and how does its tertiary structure influence interactions with FGF receptors and heparin sulfate proteoglycans? 08/27/2019

FGF8 is a member of the fibroblast growth factor family with a β-trefoil tertiary structure. This structure dictates its binding to FGF receptors and heparin sulfate proteoglycans, essential for signal transduction.

How does FGF8 participate in the crosstalk between different signaling pathways, such as Wnt and Shh, and what are the consequences for tissue morphogenesis and organogenesis? 03/18/2019

FGF8 engages in crosstalk with pathways like Wnt and Shh during tissue morphogenesis. This interaction influences organogenesis by coordinating cellular processes and ensuring proper tissue patterning.

Can you discuss the interactions between FGF8 and the extracellular matrix, and how these interactions influence the stability and bioavailability of FGF8 ligands in the tissue microenvironment? 02/05/2018

FGF8 interacts with the extracellular matrix, affecting ligand stability and bioavailability. This interaction modulates FGF8 diffusion and gradients in the tissue microenvironment.

Customer Reviews (3)

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Reviews
07/08/2021

    Top-quality and timely delivery. It's a pleasure working with this product.

    06/30/2020

      Service, quality, and shipping—all exceptional. This product is our research ally.

      10/09/2019

        Seamless shipping and a product that never disappoints. Kudos!

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