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Active Recombinant Human IL1A Protein (159 aa)

Cat.No. : IL1A-102I
Product Overview : Recombinant Human IL1A Protein without tag was expressed in E. coli.
  • Specification
  • Gene Information
  • Related Products
Description : IL-1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Source : E. coli
Species : Human
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine D10S cells is less then 0.001 ng/mL, corresponding to a Specific Activity of >1 × 10^9 IU/mg.
Molecular Mass : Approximately 18.0 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids.
Protein Length : 159
AA Sequence : SAPFSFLSNVKYNFMRIIKYEFILN DALNQSIIRANDQYLTAAALHNLDE AVKFDMGAYKSSKDDAKITVILRIS KTQLYVTAQDEDQPVLLKEMPEIPK TITGSETNLLFFWETHGTKNYFTSV AHPNLFIATKQDYWVCLAGGPPSIT DFQILENQA
Endotoxin : Less than 1 EU/μg of rHuIL-1α as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analyses.
Storage : This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.5.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20C. Further dilutions should be made in appropriate buffered solutions.
Gene Name : IL1A interleukin 1, alpha [ Homo sapiens ]
Official Symbol : IL1A
Synonyms : IL1A; interleukin 1, alpha; IL1; interleukin-1 alpha; hematopoietin 1; IL 1A; IL1 ALPHA; IL1F1; preinterleukin 1 alpha; pro interleukin 1 alpha; IL-1 alpha; hematopoietin-1; pro-interleukin-1-alpha; IL-1A; IL1-ALPHA;
Gene ID : 3552
mRNA Refseq : NM_000575
Protein Refseq : NP_000566
MIM : 147760
UniProt ID : P01583

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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