Active Recombinant Mouse Il1a Protein (Ser115-Ser270), C-His tagged, Animal-free, Carrier-free
Cat.No. : | Il1a-54M |
Product Overview : | Recombinant Mouse Il1a Protein (Ser115-Ser270) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free. |
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Description : | Interleukin-1 alpha (IL1 alpha or IL1α) is a member of the interleukin-1 cytokine family, found constitutively present in epithelial layers of the entire gastrointestinal tract, lung, liver, kidney, endothelial cells, and astrocytes. The synthesized IL-1 alpha is a 31 kDa inactive precursor and can be cleaved by intracellular caspase-1 or extracellular proteases to generate the bioactive 17 kDa form and the 16 kDa N-terminal cleavage product. Both precursor and mature IL-1 alpha protein bind to the IL-1 receptor (IL-1R), initiating a cascade of inflammatory cytokines and chemokines production such as IL-6, IL-8, and TNF, in response to viral and bacterial pathogens conditions. IL-1 alpha plays a central role in immune-surveillance mechanisms, stimulating macrophages, neutrophils, and CD8+ T cells activity. |
Source : | E. coli |
Species : | Mouse |
Tag : | His |
Form : | Lyophilized |
Bio-activity : | Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is < 5 pg/mL. The specific activity of recombinant mouse IL-1 alpha is > 2 × 10⁸ IU/mg. |
Molecular Mass : | The protein has a calculated MW of 18.93 kDa. The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis). |
Protein Length : | Ser115-Ser270 |
Endotoxin : | < 0.1 EU/μg of the protein by the LAL method. |
Purity : | >95% as determined by SDS-PAGE analysis. |
Storage : | This product is stable after storage at: -20 centigrade for 12 months in lyophilized state from date of receipt. -20 or -80 centigrade for 1 month under sterile conditions after reconstitution. Avoid repeated freeze/thaw cycles. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Reconstitution : | It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
Shipping : | The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage. |
Gene Name : | Il1a interleukin 1 alpha [ Mus musculus (house mouse) ] |
Official Symbol : | Il1a |
Synonyms : | IL1A; interleukin 1 alpha; interleukin-1 alpha; IL-1 alpha; Il-1a; |
Gene ID : | 16175 |
mRNA Refseq : | NM_010554 |
Protein Refseq : | NP_034684 |
UniProt ID : | P01582 |
Products Types
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IL1A-160P | Recombinant Active Pig IL1A Protein, His-tagged(C-ter) | +Inquiry |
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◆ Lysates | ||
IL1A-2911HCL | Recombinant Human IL1A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewHigh solubility.
Good for intracellular staining.
Effective in apoptosis assays.
Q&As (10)
Ask a questionCross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
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