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Active Recombinant Mouse Il1a Protein (Ser115-Ser270), C-His tagged, Animal-free, Carrier-free

Cat.No. : Il1a-54M
Product Overview : Recombinant Mouse Il1a Protein (Ser115-Ser270) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.
  • Specification
  • Gene Information
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Description : Interleukin-1 alpha (IL1 alpha or IL1α) is a member of the interleukin-1 cytokine family, found constitutively present in epithelial layers of the entire gastrointestinal tract, lung, liver, kidney, endothelial cells, and astrocytes. The synthesized IL-1 alpha is a 31 kDa inactive precursor and can be cleaved by intracellular caspase-1 or extracellular proteases to generate the bioactive 17 kDa form and the 16 kDa N-terminal cleavage product. Both precursor and mature IL-1 alpha protein bind to the IL-1 receptor (IL-1R), initiating a cascade of inflammatory cytokines and chemokines production such as IL-6, IL-8, and TNF, in response to viral and bacterial pathogens conditions. IL-1 alpha plays a central role in immune-surveillance mechanisms, stimulating macrophages, neutrophils, and CD8+ T cells activity.
Source : E. coli
Species : Mouse
Tag : C-His
Form : Lyophilized
Bio-activity : Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is < 5 pg/mL. The specific activity of recombinant mouse IL-1 alpha is > 2 × 10⁸ IU/mg.
Molecular Mass : The protein has a calculated MW of 18.93 kDa.
The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis).
Protein Length : Ser115-Ser270
Endotoxin : < 0.1 EU/μg of the protein by the LAL method.
Purity : >95% as determined by SDS-PAGE analysis.
Storage : This product is stable after storage at:
-20 centigrade for 12 months in lyophilized state from date of receipt.
-20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping : The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.
Gene Name : Il1a interleukin 1 alpha [ Mus musculus (house mouse) ]
Official Symbol : Il1a
Synonyms : IL1A; interleukin 1 alpha; interleukin-1 alpha; IL-1 alpha; Il-1a;
Gene ID : 16175
mRNA Refseq : NM_010554
Protein Refseq : NP_034684
UniProt ID : P01582

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.



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