Species : |
Human |
Source : |
Barley Grain |
Tag : |
His |
Description : |
Interleukin 2 (IL-2) is a potent immunoregulatory growth factor produced by T-cells following stimulation by mitogens or allogens. IL-2 promotes growth and differentiation of various cells of the immune system, such as activated T-cells and B-cells, NK cells, lymphokine activated killer cells, monocytes and macrophages. It is essential for proper immune response, it may also be an important factor in the natural suppression of autoimmunity. IL-2 may be of use as an anti-tumor agent in cancer therapy. Human and mouse IL-2 share 56% aa sequence identity and exhibit cross-species activity. |
Form : |
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2. |
Bio-activity : |
Recombinant human IL-2 is assayed by measuring its dose dependent effect on proliferation of CTLL-2 cells. The ED50 for this effect is typically< 1.25="" ng/ml,="" corresponding="" to="" a="" specific="" activity="">1 x10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines. |
Molecular Mass : |
Recombinant human IL-2 contains 133 amino acids and a 16 a.a. histidine-based tag for a total length of 149 a.a. and has a predicted molecular mass of 17.5 kDa. The recombinant protein migrates with an apparent molecular mass of 22 kDa in SDS-PAGE. |
Endotoxin : |
Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg). |
Purity : |
Greater than 95% by SDS-PAGE gel analysis. |
Storage : |
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |