Species : |
Mouse |
Source : |
E.coli |
Protein Length : |
21-169 a.a. |
Description : |
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. |
Bio-activity : |
The ED50 is 0.10-0.40 ng/ml, corresponding to a specific activity of 2.5 - 10 x 10^6 units/mg, determined by a dose dependent stimulation of HT-2 cells. |
Molecular Mass : |
17.2 kDa |
Endotoxin : |
Less than 0.01 ng per µg protein as determined by the LAL method |
Purity : |
>98%, as determined by Coomassie stained SDS-PAGE. |
Stability : |
Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
Storage : |
Store at -80 centigrade. |
Concentration : |
Protein formulated and vialed at >50 ug/mL in specified buffer |
Storage Buffer : |
5mM NaH2PO4, 5mM citric acid, 350mM NaCl, pH4.0, 1mM DTT |