Active Recombinant Human IL3

• Cat.No.: IL3-200H
• Product Overview: Recombinant human interleukin 3 expressed in CHO cells is glycosylated protein with molecular weight range from 17 to 30 kDa shown in non-reducing SDS-PAGE.

Specification

• Description: Interleukin-3 (IL-3) is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
• Source: CHO Cells
• Species: Human
• Form: Lyophilized after extensive dialysis against PBS.
• Bio-activity: ED50< 0.1 ng/ml, measured in a cell proliferation assay using tf-1 cells, corresponding to a specific activity of>1×107 units/mg.
• Molecular Mass: 17-30 kDa, observed by non-reducing SDS-PAGE.
• AA Sequence: Ala20-Phe152.
• Endotoxin: < 0.2 eu/μg, determined by lal>
• Purity: >95% as analyzed by SDS-PAGE and HPLC.
• Storage: Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-3 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.

Gene Information

• Gene Name: IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ]
• Official Symbol: IL3
• Synonyms: IL3; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; hematopoietic growth factor; IL 3; mast cell growth factor; MCGF; MGC79398; MGC79399; MULTI CSF; multilineage colony stimulating factor; P cell stimulating factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; IL-3; MULTI-CSF
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700
• Chromosome Location: 5q23-q31
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem
• Function: cytokine activity; growth factor activity; interleukin-3 receptor binding