| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Non |
| Protein Length : |
20-152 a.a. |
| Description : |
Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) that can improve the body"s natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 . Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats, and the PI3K/Akt pathway. IL-3 may play an important role in the development of airway inflammation associated with asthma. The human IL-3 gene encodes a protein 152 amino acids long, and the naturally occurring IL-3 is glycosylated. The human IL-3 gene is located on chromosome 5, only 9 kilobases from the GM-CSF gene, and its function is quite similar to GM-CSF. |
| Predicted N Terminal : |
Ala20 |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
| Bio-activity : |
The bio-activity was determined by its ability to induce IFN-γ production by NK-92 cells. The ED50<0.1 ng/ml,="" corresponding="" to="" a="" specific="" activity="" of="">1X10^7 Unit/mg. |
| Molecular Mass : |
rhIL3, a 133 amino acids protein, and has a calculated MW of 15 kDa. DTT-reduced protein migrates as 30 and 38 kDa monomer due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU per μg of the rhIL3 by the LAL method. |
| Purity : |
>92% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade-8 centigrade); After reconstitution under sterile conditions for 1 month (4 centigrade-8 centigrade) or 3 months (-20 centigrade to -70 centigrade). |
