Active Recombinant Human Interleukin-11
| Cat.No. : | IL11-416H |
| Product Overview : | Interleukin-11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19256.29 Dalton. The IL-11 is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
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| Cat. No. : | IL11-416H |
| Description : | IL11 is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). IL-11 is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. |
| Source : | Human |
| Host : | Escherichia Coli. |
| Form : | The protein was lyophilized from a concentrated (1mg/ml) solution with no additives. |
| Purity : | Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Bio-activity : | The ED50 as determined by the dose-dependant stimulation of the proliferation of murine 7TD1 was found to be < 10ng/ml, corresponding to a Specific Activity of 100,000 IU/mg. |
| Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility : | It is recommended to reconstitute the lyophilized Interleukin 11 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Amino acid sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Pro-Pro-Gly.N-terminal methionine has been completely removed enzymatically. |
| Protein content : | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.95 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-11 as a Reference Standard. |
| Storage : | Lyophilized Interleukin-11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Gene Name : | IL11 interleukin 11 [ Homo sapiens ] |
| Official Symbol : | IL11 |
| Synonyms : | AGIF; IL-11; Adipogenesis inhibitory factor, Oprelvekin, Interleukin-11; IL11; interleukin 11 |
| Gene ID : | 3589 |
| mRNA Refseq : | NM_000641 |
| Protein Refseq : | NP_000632 |
| UniProt ID : | P20809 |
| Chromosome Location : | 19q13.3-q13.4 |
| MIM : | 147681 |
| Pathway : | Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway |
| Function : | cytokine activity, interleukin-11 receptor binding activity |
Products Types
| ◆ Recombinant Protein | ||
| IL11-2016H | Recombinant Human IL11 Protein, Met-tagged | +Inquiry |
| IL11-061H | Active Recombinant Human IL11 Protein | +Inquiry |
| Il11-085M | Active Recombinant Mouse Il11 Protein | +Inquiry |
| IL11-175H | Active Recombinant Human IL11 Protein (Pro22-Leu199), N-His tagged, Animal-free, Carrier-free | +Inquiry |
| IL11-130H | Active Recombinant Human IL11 Protein | +Inquiry |
| ◆ Lysates | ||
| IL11-5249HCL | Recombinant Human IL11 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (10)
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How are in vivo models, including transgenic mice and knockdown strategies, utilized to study IL11's effects on tissue regeneration and homeostasis?
11/05/2022
Transgenic mice and knockdown models explore IL11's impact on tissue regeneration.
Could you explain the experimental approaches, such as site-directed mutagenesis, employed to identify key residues in IL11's interaction with IL11RA?
07/14/2021
Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.
What techniques like chromatin immunoprecipitation and DNA methylation analysis contribute to deciphering IL11's regulatory mechanisms?
05/27/2021
Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.
Can you elaborate on the role of proteomics in identifying IL11-interacting proteins and potential cross-talk with other signaling pathways?
09/28/2020
Proteomics identifies IL11-interacting proteins and potential cross-talk partners.
How do cell-based assays and luciferase reporter assays help investigate IL11's downstream signaling pathways through JAK-STAT activation?
05/19/2020
Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.
What role does RNA sequencing play in understanding IL11-induced gene expression changes in various cell types?
02/24/2019
RNA sequencing uncovers IL11-induced gene expression changes across different cell types.
What structural insights have X-ray crystallography and nuclear magnetic resonance spectroscopy provided about the IL11-IL11RA complex?
01/13/2019
X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.
How do advanced imaging techniques like confocal microscopy and super-resolution microscopy visualize IL11 localization and distribution in tissues?
11/08/2018
Confocal and super-resolution microscopy visualize IL11 localization in tissues.
How are techniques like surface plasmon resonance used to study IL11's binding kinetics to its receptor IL11RA?
04/17/2017
Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.
What insights have CRISPR screening approaches provided regarding genes and pathways influenced by IL11 signaling?
02/06/2017
CRISPR screening unveils genes and pathways influenced by IL11 signaling.
Customer Reviews (3)
Write a reviewReviews
10/28/2019
Potent in bone regeneration models. -
11/26/2017
Suitable for cell culture. -
01/10/2017
High yield in expression. -
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