| Species : |
Mouse |
| Source : |
Mouse |
| Tag : |
Non |
| Description : |
Il11 issecreted by bone marrow stromal cells (fibroblasts)and is produced also bya number of mesenchymal cells. The Il11receptor (alpha chain) utilizes gp130 as its signal transducer, which isalso a component of other cytokine receptors. The alpha chain of the Il11receptor has been identified independently as Etl2. Il11promotes primary and secondary immune responses in vitro and in vivo andmodulates antigen-specific antibody reactions. |
| Purity : |
>95%, asdetermined by SDS-PAGE and HPLC. |
| Sequence : |
MNCVCRLVLV VLSLWPDRVV APGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL |
| Molecular Weight : |
Native MouseIl11, generated by the proteolytic removal of the signal peptide andpropeptide, the molecule has a calculated molecular mass of approximately 19kDa. |
| Biological Activity : |
The ED(50) wasdetermined by the dose-dependent stimulation of the proliferation of murineT11 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106units/mg. |
| Endotoxin : |
Endotoxin contentwas assayed using a LAL gel clot method. Endotoxin level was found to be lessthan 0.1 ng/µg(1EU/µg). |
| Reconstitution : |
A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage : |
The lyophilized protein is stable forat least 2 years from date of receipt at -20°C. Upon reconstitution, thiscytokine can be stored in working aliquots at 2°C - 8°C for one month, or at-20°C for six months, with a carrier protein without detectable loss ofactivity. Avoid repeated freeze/thaw cycles. |
| OfficialSymbol : |
Il11 |
| Pathways : |
Cytokine-cytokinereceptor interaction; Cytokines and Inflammatory Response (BioCarta); Hematopoieticcell lineage; Jak-STAT signaling pathway; Rheumatoid arthritis |
