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Recombinant Mouse interleukin 11

Cat.No. : Il11-586M
Product Overview : RecombinantIl11 is a disulfide-linked monomer protein consisting of 179 amino acidresidue subunits, and migrates as an approximately 19 kDa protein undernon-reducing conditions and reducing conditions in SDS-PAGE, and waslyophilized from a 0.2 μm filtered PBS solution.
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Cat. No. : Il11-586M
Description : Il11 issecreted by bone marrow stromal cells (fibroblasts)and is produced also bya number of mesenchymal cells. The Il11receptor (alpha chain) utilizes gp130 as its signal transducer, which isalso a component of other cytokine receptors. The alpha chain of the Il11receptor has been identified independently as Etl2. Il11promotes primary and secondary immune responses in vitro and in vivo andmodulates antigen-specific antibody reactions.
Source : Mouse
Host : E. coli
Purity : >95%, asdetermined by SDS-PAGE and HPLC.
Sequence : MNCVCRLVLV VLSLWPDRVV APGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL
Molecular Weight : Native MouseIl11, generated by the proteolytic removal of the signal peptide andpropeptide, the molecule has a calculated molecular mass of approximately 19kDa.
Biological Activity : The ED(50) wasdetermined by the dose-dependent stimulation of the proliferation of murineT11 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106units/mg.
Endotoxin : Endotoxin contentwas assayed using a LAL gel clot method. Endotoxin level was found to be lessthan 0.1 ng/µg(1EU/µg).
Reconstitution : A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage : The lyophilized protein is stable forat least 2 years from date of receipt at -20°C. Upon reconstitution, thiscytokine can be stored in working aliquots at 2°C - 8°C for one month, or at-20°C for six months, with a carrier protein without detectable loss ofactivity. Avoid repeated freeze/thaw cycles.
OfficialSymbol : Il11
Pathways : Cytokine-cytokinereceptor interaction; Cytokines and Inflammatory Response (BioCarta); Hematopoieticcell lineage; Jak-STAT signaling pathway; Rheumatoid arthritis
Gene Name : Il11 interleukin 11 [ Mus musculus ]
Synonyms : Il11; interleukin11; IL-11; interleukin-11
Gene ID : 16156
mRNA Refseq : NM_008350
Protein Refseq : NP_032376
UniProt ID : P47873
Function : cytokine activity; growthfactor activity; interleukin-11 receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How are in vivo models, including transgenic mice and knockdown strategies, utilized to study IL11's effects on tissue regeneration and homeostasis? 11/05/2022

Transgenic mice and knockdown models explore IL11's impact on tissue regeneration.

Could you explain the experimental approaches, such as site-directed mutagenesis, employed to identify key residues in IL11's interaction with IL11RA? 07/14/2021

Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.

What techniques like chromatin immunoprecipitation and DNA methylation analysis contribute to deciphering IL11's regulatory mechanisms? 05/27/2021

Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.

Can you elaborate on the role of proteomics in identifying IL11-interacting proteins and potential cross-talk with other signaling pathways? 09/28/2020

Proteomics identifies IL11-interacting proteins and potential cross-talk partners.

How do cell-based assays and luciferase reporter assays help investigate IL11's downstream signaling pathways through JAK-STAT activation? 05/19/2020

Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.

What role does RNA sequencing play in understanding IL11-induced gene expression changes in various cell types? 02/24/2019

RNA sequencing uncovers IL11-induced gene expression changes across different cell types.

What structural insights have X-ray crystallography and nuclear magnetic resonance spectroscopy provided about the IL11-IL11RA complex? 01/13/2019

X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.

How do advanced imaging techniques like confocal microscopy and super-resolution microscopy visualize IL11 localization and distribution in tissues? 11/08/2018

Confocal and super-resolution microscopy visualize IL11 localization in tissues.

How are techniques like surface plasmon resonance used to study IL11's binding kinetics to its receptor IL11RA? 04/17/2017

Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.

What insights have CRISPR screening approaches provided regarding genes and pathways influenced by IL11 signaling? 02/06/2017

CRISPR screening unveils genes and pathways influenced by IL11 signaling.

Customer Reviews (3)

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Reviews
10/28/2019

    Potent in bone regeneration models.

    11/26/2017

      Suitable for cell culture.

      01/10/2017

        High yield in expression.

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