Recombinant Human Interleukin 11
Cat.No. : | IL11-218H |
Product Overview : | Recombinant Human interleukin 11 DNA sequence encoding the mature 173 ammino acid human IL-11 protein sequence was inserted into a suitable expression vector and was expressed inSf21 insect cellsusing a baculovirus expression system. |
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Cat. No. : | IL11-218H |
Description : | Interleukin 11 (IL-11) is a multifunctional cytokine isolated from bone marrow-derived stromal cells. It is a key regulator of multiple events in hemtaopoiesis, most notably the stimulation of megakaryocyte maturation. It is also known under the names Adipogenesis inhibitory factor (AGIF) and Oprelvekin. IL-11 improves platelet recovery after chemotherapy-induced thrombocytopenia, induces acute phase proteins, modulates antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis. |
Source : | Sf21 Insect Cells. |
Molecular Weight : | The predicted molecular weight of Recombinant Human IL-11 is Mr 19 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 23 kDa. |
State Of Matter : | Solution. |
Formulation : | This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present. The final protein concentration is 0.25 mg/ml. |
Purity : | > 97% by SDS Page and analyzed by silver stain. |
Endotoxin : | < 0.1 ng per 1 µg as determined by the LAL method. |
Biological Activity : | The biological activity of Human IL-11 was determined by a cell proliferation assay using T11, a subline of the IL-6-dependent murine plasmacytoma cell line T1165.85.2.1 that has been adapted to grow in IL-11. The expected ED50 for this effect is typically 0.06 - 0.24 ng/mL. |
Storage And Stability : | Working aliquots of this recombinant protein solution are stable for up to twelve months at -20˚C to -70˚C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2˚C - 8˚C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20˚C to -70˚C in a manual defrost freezer. Avoid repeated freeze thaw cycles. |
Tag : | Non |
Gene Name : | IL11 interleukin 11 [ Homo sapiens ] |
Synonyms : | AGIF; IL-11; Adipogenesis inhibitory factor, Oprelvekin, Interleukin-11; IL11; interleukin 11 |
Gene ID : | 3589 |
mRNA Refseq : | NM_000641 |
Protein Refseq : | NP_000632 |
UniProt ID : | P20809 |
Chromosome Location : | 19q13.3-q13.4 |
MIM : | 147681 |
Pathway : | Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway |
Function : | cytokine activity, interleukin-11 receptor binding activity |
Products Types
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IL11-2016H | Recombinant Human IL11 Protein, Met-tagged | +Inquiry |
Il11-842M | Recombinant Mouse Il11 protein(Pro22-Leu199) | +Inquiry |
IL11-1305C | Recombinant Cynomolgus IL11 protein(Phe22-Leu199) | +Inquiry |
◆ Lysates | ||
IL11-5249HCL | Recombinant Human IL11 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewPotent in bone regeneration models.
Suitable for cell culture.
High yield in expression.
Q&As (10)
Ask a questionTransgenic mice and knockdown models explore IL11's impact on tissue regeneration.
Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.
Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.
Proteomics identifies IL11-interacting proteins and potential cross-talk partners.
Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.
RNA sequencing uncovers IL11-induced gene expression changes across different cell types.
X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.
Confocal and super-resolution microscopy visualize IL11 localization in tissues.
Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.
CRISPR screening unveils genes and pathways influenced by IL11 signaling.
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