| Species : |
Human |
| Source : |
E.coli |
| Protein Length : |
181 amino acid |
| Description : |
The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Bio-activity : |
Human LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1 × 10^6 units/ml and 1 × 10^7 units/ml, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL of medium. |
| Molecular Mass : |
20 kDa |
| Endotoxin : |
< 0.01 ng/μg cytokine as determined by the LAL assay |
| Purity : |
> 97% by SDS-PAGE |
| Usage : |
1 mL of human LIF with the specific activity of 1×10^6 units/ml is sufficient to treat 1 L of embryonic stem cell culture media. 1 ml of human LIF with the specific activity of 1×10^7 units/ml is sufficient to treat 10 L of embryonic stem cell culture media. |
| Storage : |
It is stable for 6 to 12 months from date of receipt when stored at 2-4 centigrade. For extended storage, it is recommended to store in working aliquots at -20 to -80 centigrade. Freeze-thaw should be avoided as it can result in loss of activity. |
| Storage Buffer : |
1×phosphate-buffered saline with 0.02% Tween-20. Sterile filtered through a 0.2 micron filter. |
| Shipping : |
This product is shipped at ambient temperature with cold pack. |
