Species : |
Human |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Met1-Asn469 |
Form : |
Lyophilized from sterile 25mM MES, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 5.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >400 pmoles/min/μg(Activation description: The proenzyme needs to be activated by APMA for an activated form) |
Molecular Mass : |
The secreted recombinant human MMP1 comprises 461 amino acids migrates as an approximately 50-55 kDa band as estimated in SDS-PAGE under reducing conditions. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : |
> 95 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |