| Description : |
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer. |
| Source : |
HEK293 |
| Species : |
Human |
| Predicted N Terminal : |
Leu 25 |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
| Bio-activity : |
Immobilized rh full length PDCD1 /PD1 at 2 μg/ml ( 100 μl/well ) can bind Human PD-L1 /B7-H1 Fc Chimera with a linear range of 20 - 200 ng/ml. |
| Molecular Mass : |
DTT-reduced Protein migrates as 29-70 kDa in SDS-PAGE due to glycosylation. |
| Protein length : |
Leu 25 - Leu 288 |
| Endotoxin : |
Less than 1.0 EU per μg of the rh PDCD1 /PD1 (full length) by the LAL method. |
| Purity : |
>92% as determined by SDS-PAGE. |
| Storage : |
No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade). |
| Warning : |
Avoid repeated freeze-thaw cycles. |
