| Source : |
HEK293 |
| Species : |
Cynomolgus |
| Tag : |
His-Avi |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Biotinylated Cynomolgus PD-1, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Cynomolgus / Rhesus macaque PD-L1, Fc Tag with a linear range of 0.2-5 ng/mL. Immobilized Biotinylated Cynomolgus PD-1, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Nivolumab with a linear range of 1-20 ng/mL. Immobilized Human PD-L2, Fc Tag (HPLC verified) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus PD-1, His,Avitag with a linear range of 0.039-1.25 μg/mL. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 19.5 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Proteinlength : |
Leu25-Gln167 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
