Active Recombinant Human SIRT1, His-tagged

• Cat.No.: SIRT1-1360H
• Product Overview: Recombinant human SIRT1 (193-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Description: SIRT1 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein. Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation.
• Source: Sf9 Cells
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Bio-activity: 2100 RLU/min/ng
• Molecular Mass: ~84 kDa
• Protein length: 193 aa-end
• Purity: >70%
• Applications: SIRT Assay
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: SIRT1 sirtuin 1 [ Homo sapiens ]
• Official Symbol: SIRT1
• Synonyms: SIRT1; sirtuin 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1; SIR2L1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1
• Gene ID: 23411
• mRNA Refseq: NM_001142498
• Protein Refseq: NP_001135970
• MIM: 604479
• UniProt ID: Q96EB6
• Chromosome Location: 10q21
• Pathway: Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; E2F transcription factor network, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem
• Function: HLH domain binding; NOT NAD+ ADP-ribosyltransferase activity; NAD+ binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent protein deacetylase activity; NAD-dependent protein deacetylase activity; bHLH transcription factor binding; deacetylase activity; enzyme binding; histone binding; histone deacetylase activity; identical protein binding; metal ion binding; mitogen-activated protein kinase binding; p53 binding; protein C-terminus binding; protein binding; protein deacetylase activity; transcription corepressor activity; transcription corepressor activity; zinc ion binding

Reviews

10/12/2021

Resulting banding is clear and bright, much more than expected.

10/09/2021

SIRT1 has high safety, environmental protection and no pollution.

01/03/2021

The quality is so good. It saves a lot of time. Good.

Q&As

Does SIRT1 play a role in tissue regeneration?

Studies have found that SIRT1 plays an important role in tissue regeneration. Activation of SIRT1 can promote the proliferation and differentiation of stem cells, which contributes to tissue regeneration and repair.

Is SIRT1 related to autophagy?

Activation of SIRT1 promotes autophagy, which helps to remove damaged organelles and proteins and maintain cell health.

What is the relationship between SIRT1 and mitochondrial function?

SIRT1 regulates mitochondrial function. Activation of SIRT1 can improve mitochondrial metabolism and function, contributing to energy metabolism and cell survival.

What role does SIRT1 play in cancer?

Studies have found that SIRT1 exerts carcinogenic effects in some cancers, but exerts anti-cancer effects in others. It depends on the type of cancer and environmental factors.

What is the potential of SIRT1 in drug development?

SIRT1 has potential therapeutic value in drug development. The development of small molecule drugs targeting SIRT1 can be used to treat a variety of diseases, including aging, diabetes, cardiovascular diseases, and neurodegenerative diseases.

How does SIRT1 relate to other sirtuins?

SIRT1 interacts and regulates with other sirtuins. For example, SIRT1 can regulate the expression and activity of other Sirtuins, and other Sirtuins can also regulate the activity of SIRT1.