Recombinant Human SIRT1 Protein, GST-tagged SIRT1-30H

Recombinant Human SIRT1 Protein, GST-tagged

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Recombinant Human SIRT1 Protein, GST-tagged

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Cat.No. : SIRT1-30H
Product Overview : Recombinant human SIRT1(193-747) protein with a N-terminal GST-tag was expressed in E. coli.
Description : This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Source : E. coli
Species : Human
Tag : GST
Molecular Mass : 89.2 kDa
Protein length : 193-747
Purity : ≥70% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 pmole of 7-amino-4-methylcoumarin per minute at 25 centigrade in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, containing 125 μM p53 peptide amino acids 379-382 (Arg-His-Lys-Lys (e-acetyl)-AMC), and 3 mM NAD+.
Stability : ≥ 1 year
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM sodium phosphate, pH 7.2, with 100 mM sodium chloride and 20% glycerol
SDS-PAGE Analysis of SIRT1 :

Lane 1: MW Markers; Lane 2: SIRT1 (2 μg); Lane 3: SIRT1 (4 μg). Representative gel image shown; actual purity may vary between each batch.

Inhibition of SIRT1 by Sirtinol (IC50 = 38 μM) :

SIRT1 Activity Assay :

Gene Name : SIRT1 sirtuin 1 [ Homo sapiens (human) ]
Official Symbol : SIRT1
Synonyms : SIRT1; sirtuin 1; SIR2; SIR2L1; SIR2alpha; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacylase sirtuin-1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; sirtuin type 1
Gene ID : 23411
mRNA Refseq : NM_001142498
Protein Refseq : NP_001135970
MIM : 604479
UniProt ID : Q96EB6

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