Species : |
Human |
Source : |
E.coli |
Tag : |
GST |
Protein Length : |
193-747 |
Description : |
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. |
Molecular Mass : |
89.2 kDa |
Purity : |
≥70% estimated by SDS-PAGE |
Unit Definition : |
One unit is defined as the amount of enzyme required to produce 1 pmole of 7-amino-4-methylcoumarin per minute at 25 centigrade in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, containing 125 μM p53 peptide amino acids 379-382 (Arg-His-Lys-Lys (e-acetyl)-AMC), and 3 mM NAD+. |
Stability : |
≥ 1 year |
Storage : |
Store it at -80 centigrade |
Storage Buffer : |
50 mM sodium phosphate, pH 7.2, with 100 mM sodium chloride and 20% glycerol |