Recombinant Human SIRT1 Protein, GST-tagged


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Recombinant Human SIRT1 Protein, GST-tagged

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Cat.No. : SIRT1-30H
Product Overview : Recombinant human SIRT1(193-747) protein with a N-terminal GST-tag was expressed in E. coli.
Description : This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Source : E. coli
Species : Human
Tag : GST
Molecular Mass : 89.2 kDa
Protein length : 193-747
Purity : ≥70% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 pmole of 7-amino-4-methylcoumarin per minute at 25 centigrade in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, containing 125 μM p53 peptide amino acids 379-382 (Arg-His-Lys-Lys (e-acetyl)-AMC), and 3 mM NAD+.
Stability : ≥ 1 year
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM sodium phosphate, pH 7.2, with 100 mM sodium chloride and 20% glycerol
SDS-PAGE Analysis of SIRT1 :

Lane 1: MW Markers; Lane 2: SIRT1 (2 μg); Lane 3: SIRT1 (4 μg). Representative gel image shown; actual purity may vary between each batch.

Inhibition of SIRT1 by Sirtinol (IC50 = 38 μM) :

SIRT1 Activity Assay :

Gene Name : SIRT1 sirtuin 1 [ Homo sapiens (human) ]
Official Symbol : SIRT1
Synonyms : SIRT1; sirtuin 1; SIR2; SIR2L1; SIR2alpha; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacylase sirtuin-1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; sirtuin type 1
Gene ID : 23411
mRNA Refseq : NM_001142498
Protein Refseq : NP_001135970
MIM : 604479
UniProt ID : Q96EB6
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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