Active Recombinant Human SRC protein, His-tagged

• Cat.No.: SRC-533H
• Product Overview: Recombinant Human SRC fused with His tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Form: 25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT
• Bio-activity: 205 pmol/min/μg
• Molecular Mass: 60.5 kDa
• Purity: >85%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 1.2 mg/ml
• Publications:
  C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src. (2017)

Gene Information

• Gene Name: SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens ]
• Official Symbol: SRC
• Synonyms: ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• Gene ID: 6714
• mRNA Refseq: NM_005417
• Protein Refseq: NP_005408
• MIM: 190090
• UniProt ID: P12931

Citation

C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src

Journal: Molecular Biology of the Cell    Data: 2018/1/15

Authors: G. D. N. Gayathri Samarasekera, Vanessa Jane Auld

Article Snippet:A 20 μl Kinase Reaction [0.5 μg His tagged GliCter in pET28, 0.5 μg GST–Csk (Cell Signaling Technologies), 1x Kinase Buffer, 1 mM ATP] was incubated at 30°C for 30 minutes.A 20 μl Kinase Reaction [0.5 μg His tagged GliCter in pET28, 0.5 μg GST–Csk (Cell Signaling Technologies), 1x Kinase Buffer, 1 mM ATP] was incubated at 30°C for 30 minutes.. In the control experiment 0.5 μg of His tagged human Src (Creative BioMart) was used.. Li-cor western blot analysis Half of the kinase reaction was loaded on 10% SDS-PAGE followed by western blot on nitrocellulose membranes.Li-cor western blot analysis Half of the kinase reaction was loaded on 10% SDS-PAGE followed by western blot on nitrocellulose membranes.