Active Recombinant Human SRC protein, His-tagged

• Cat.No.: SRC-533H
• Product Overview: Recombinant Human SRC fused with His tag at N-terminal was expressed in Insect cells.

Specification

• Description: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: 25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT
• Bio-activity: 205 pmol/min/μg
• Molecular Mass: 60.5 kDa
• Purity: >85%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 1.2 mg/ml

Gene Information

• Gene Name: SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens ]
• Official Symbol: SRC
• Synonyms: ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• Gene ID: 6714
• mRNA Refseq: NM_005417
• Protein Refseq: NP_005408
• MIM: 190090
• UniProt ID: P12931

Citation

C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src

Journal: Molecular Biology of the Cell    Data: 2018/1/15

Authors: G. D. N. Gayathri Samarasekera, Vanessa Jane Auld

Article Snippet:A 20 μl Kinase Reaction [0.5 μg His tagged GliCter in pET28, 0.5 μg GST–Csk (Cell Signaling Technologies), 1x Kinase Buffer, 1 mM ATP] was incubated at 30°C for 30 minutes.A 20 μl Kinase Reaction [0.5 μg His tagged GliCter in pET28, 0.5 μg GST–Csk (Cell Signaling Technologies), 1x Kinase Buffer, 1 mM ATP] was incubated at 30°C for 30 minutes.. In the control experiment 0.5 μg of His tagged human Src (Creative BioMart) was used.. Li-cor western blot analysis Half of the kinase reaction was loaded on 10% SDS-PAGE followed by western blot on nitrocellulose membranes.Li-cor western blot analysis Half of the kinase reaction was loaded on 10% SDS-PAGE followed by western blot on nitrocellulose membranes.

Publication :

C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src. (2017)

Reviews

01/01/2022

Top-notch protein purification service, essential for our research.

08/29/2019

Reliable mass spectrometry analysis, pivotal for our proteomics work.

03/02/2019

Swift and reliable protein sequencing, indispensable for our research success.

Q&As

How do alterations in SRC expression or activity affect the development of drug resistance in tumors?

Changes in SRC expression or kinase activity can lead to drug resistance in cancer treatment.

How does SRC (proto-oncogene tyrosine-protein kinase Src) regulate cell proliferation and survival?

SRC regulates cell proliferation and survival through its kinase activity, influencing various signaling pathways.

How does SRC contribute to the regulation of bone remodeling and osteoclast activity?

SRC is involved in bone remodeling, regulating osteoclast activity and bone resorption.

What role does SRC play in cancer development and progression, particularly in metastasis?

SRC plays a crucial role in cancer progression, particularly in enhancing metastatic potential of tumor cells.

How does SRC influence cellular signaling pathways, such as the Ras/MAPK and PI3K/Akt pathways?

SRC influences key cellular signaling pathways like Ras/MAPK and PI3K/Akt, affecting cell growth and survival.

What are the mechanisms by which SRC modulates cellular adhesion and migration?

SRC modulates cellular adhesion and migration, playing a role in tissue repair and cancer metastasis.

What is the impact of SRC inhibition on targeted cancer therapies?

SRC inhibition is a target in cancer therapy, potentially reducing tumor growth and metastasis.