Active Recombinant Human SRC protein, His-tagged
Cat.No. : | SRC-533H |
Product Overview : | Recombinant Human SRC fused with His tag at N-terminal was expressed in Insect cells. |
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Description : | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
Source : | Insect cells |
Species : | Human |
Tag : | His |
Form : | 25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT |
Bio-activity : | 205 pmol/min/μg |
Molecular Mass : | 60.5 kDa |
Purity : | >85% |
Applications : | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Storage : | >6 months at –80 centigrade. Avoid freeze/thaw cycles. |
Concentration : | 1.2 mg/ml |
Gene Name : | SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens ] |
Official Symbol : | SRC |
Synonyms : | ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog |
Gene ID : | 6714 |
mRNA Refseq : | NM_005417 |
Protein Refseq : | NP_005408 |
MIM : | 190090 |
UniProt ID : | P12931 |
Products Types
◆ Recombinant Protein | ||
SRC-2094H | Recombinant Human SRC Protein, His (Fc)-Avi-tagged | +Inquiry |
SRC-2747H | Recombinant Human SRC Protein, MYC/DDK-tagged | +Inquiry |
SRC-0402H | Recombinant Human SRC Protein (G2-L536), Tag Free | +Inquiry |
SRC-4276R | Recombinant Rhesus Macaque SRC Protein, His (Fc)-Avi-tagged | +Inquiry |
SRC-004H | Active Recombinant Human SRC Protein, GST-tagged | +Inquiry |
◆ Native Protein | ||
SRC-29697TH | Native Human SRC | +Inquiry |
◆ Lysates | ||
SRC-585MCL | Recombinant Mouse SRC cell lysate | +Inquiry |
SRC-487HCL | Recombinant Human SRC cell lysate | +Inquiry |
Related Gene
C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src
Journal: Molecular Biology of the Cell Data: 2018/1/15
Authors: G. D. N. Gayathri Samarasekera, Vanessa Jane Auld
Article Snippet:A 20 μl Kinase Reaction [0.5 μg His tagged GliCter in pET28, 0.5 μg GST–Csk (Cell Signaling Technologies), 1x Kinase Buffer, 1 mM ATP] was incubated at 30°C for 30 minutes.A 20 μl Kinase Reaction [0.5 μg His tagged GliCter in pET28, 0.5 μg GST–Csk (Cell Signaling Technologies), 1x Kinase Buffer, 1 mM ATP] was incubated at 30°C for 30 minutes.. In the control experiment 0.5 μg of His tagged human Src (Creative BioMart) was used.. Li-cor western blot analysis Half of the kinase reaction was loaded on 10% SDS-PAGE followed by western blot on nitrocellulose membranes.Li-cor western blot analysis Half of the kinase reaction was loaded on 10% SDS-PAGE followed by western blot on nitrocellulose membranes.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionChanges in SRC expression or kinase activity can lead to drug resistance in cancer treatment.
SRC regulates cell proliferation and survival through its kinase activity, influencing various signaling pathways.
SRC is involved in bone remodeling, regulating osteoclast activity and bone resorption.
SRC plays a crucial role in cancer progression, particularly in enhancing metastatic potential of tumor cells.
SRC influences key cellular signaling pathways like Ras/MAPK and PI3K/Akt, affecting cell growth and survival.
SRC modulates cellular adhesion and migration, playing a role in tissue repair and cancer metastasis.
SRC inhibition is a target in cancer therapy, potentially reducing tumor growth and metastasis.
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