| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Non |
| Protein Length : |
77-233 a.a. |
| Description : |
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Form : |
Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally 5-8% trehalose and mannitol are added as protectants before lyophilization. |
| Bio-activity : |
The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D. ED50<25pg/ml, corresponding to a specific activity of >4X10^7 Unit/mg |
| Molecular Mass : |
rhTNFa, without any tag, has a calculated MW of 17.4 kDa expressed. The predicted N-terminus is Val77. Protein migrates as 18 kDa in reduced SDS-PAGE. The gel filtration analysis indicates that only HEK293 expressed TNF alpha protein is trimeric form, which has a molecular mass of 54 kDa. |
| Endotoxin : |
Less than 1.0 EU per 1 μg of the rhTNFa by the LAL method. |
| Purity : |
>98% as determined by SDS-PAGE. All lots are greater than 95% pure. |
| Storage : |
Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhTNFa should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles. |
