| Cat.No. : |
TNF-39H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant human TNF alpha protein (rhTNFa) Val77-Leu233 was produced in human HEK293 cells. |
| Description : |
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Source : |
HEK293 cells |
| Species : |
Human |
| Form : |
Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally 5-8% trehalose and mannitol are added as protectants before lyophilization. |
| Bio-activity : |
The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D. ED50<25pg/ml, corresponding to a specific activity of >4X10^7 Unit/mg |
| Molecular Mass : |
rhTNFa, without any tag, has a calculated MW of 17.4 kDa expressed. The predicted N-terminus is Val77. Protein migrates as 18 kDa in reduced SDS-PAGE. The gel filtration analysis indicates that only HEK293 expressed TNF alpha protein is trimeric form, which has a molecular mass of 54 kDa. |
| Endotoxin : |
Less than 1.0 EU per 1 μg of the rhTNFa by the LAL method. |
| Purity : |
>98% as determined by SDS-PAGE. All lots are greater than 95% pure. |
| Storage : |
Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhTNFa should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
