Active Recombinant Human TNFa

• Cat.No.: TNF-39H
• Product Overview: Recombinant human TNF alpha protein (rhTNFa) Val77-Leu233 was produced in human HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 77-233 a.a.
• Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Form: Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder.Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D. ED50<25pg/ml, corresponding to a specific activity of >4X10^7 Unit/mg
• Molecular Mass: rhTNFa, without any tag, has a calculated MW of 17.4 kDa expressed. The predicted N-terminus is Val77. Protein migrates as 18 kDa in reduced SDS-PAGE. The gel filtration analysis indicates that only HEK293 expressed TNF alpha protein is trimeric form, which has a molecular mass of 54 kDa.
• Endotoxin: Less than 1.0 EU per 1 μg of the rhTNFa by the LAL method.
• Purity: >98% as determined by SDS-PAGE. All lots are greater than 95% pure.
• Storage: Store at -20? in lyophilized state after receipt. For long term storage, upon reconstitution rhTNFa should be aliquot and store at -20? or -80?. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); DIF; TNFA; TNFSF2; TNF-alpha; Cachectin; OTTHUMP00000037669; TNF-a; APC1 protein; TNF superfamily, member 2; TNF, macrophage-derived; TNF, monocyte-derived; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor precursor; tumor necrosis factor (cachectin); tumor necrosis factor alpha; tumor necrosis factor, alpha (cachectin)
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375
• Chromosome Location: 6p21.3
• Pathway: Adipocytokine signaling pathway; AhR pathway; Alzheimer's disease
• Function: Cytokine activity; Identical protein binding; Tumor necrosis factor receptor binding