Recombinant Human TNFA Protein, Met-tagged, Biotinylated

• Cat.No.: TNFA-344H
• Product Overview: Biotinylated Recombinant Human TNFA Protein(NP_000585.2) (Val77-Leu233) was expressed in E. coli, fused with an initial Met at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Val77-Leu233
• Form: Lyophilized from sterile Sterile PBS.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human TNF consists of 158 amino acids and predicts a molecular mass of 17.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin