Active Recombinant Human USP6, GST-tagged

• Cat.No.: USP6-154H
• Product Overview: Recombinant human USP6 (amino acid residues 529-1406), fused with N-terminal GST, was expressed in Insect (Sf21).

Specification

• Description: The deubiquitylating – or deubiquitinating – enzymes (DUBs) represent the largest family of DCEs and regulate ubiquitin dependent signalling pathways. The activities of the DUBs include the generation of free ubiquitin from precursor molecules, the recycling of ubiquitin following substrate degradation to maintain cellular ubiquitin homeostasis and the removal of ubiquitin or ubiquitin-like proteins (UBL) modifications through chain editing to rescue proteins from proteasomal degradation or to influence cell signalling events (Komander et al., 2009).
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-USP6 CD(529-1406) was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-USP6 CD(529-1406) was compared confirming the deubiquitylating activity of GST-USP6 CD(529-1406).
• Molecular Mass: ~125.3kDa
• Purity: >88% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: USP6 ubiquitin specific peptidase 6 (Tre-2 oncogene) [ Homo sapiens ]
• Official Symbol: USP6
• Synonyms: USP6; ubiquitin specific peptidase 6 (Tre-2 oncogene); HRP1, ubiquitin specific protease 6 (Tre 2 oncogene); ubiquitin carboxyl-terminal hydrolase 6; TBC1D3 and USP32 fusion; Tre 2; Tre2; TRE17; ubiquitin carboxyl terminal hydrolase 6; tre-2 oncogene; proto-oncogene TRE-2; hyperpolymorphic gene 1; ubiquitin thioesterase 6; deubiquitinating enzyme 6; ubiquitin thiolesterase 6; ubiquitin specific peptidase 6-; ubiquitin-specific protease USP6; ubiquitin-specific-processing protease 6; ubiquitin specific protease 6 (Tre-2 oncogene); HRP1; TRE2; Tre-2; USP6-short
• Gene ID: 9098
• mRNA Refseq: NM_004505
• Protein Refseq: NP_004496
• MIM: 604334
• UniProt ID: P35125
• Chromosome Location: 17p13
• Pathway: Arf6 signaling events, organism-specific biosystem
• Function: Rab GTPase activator activity; calmodulin binding; cysteine-type endopeptidase activity; nucleic acid binding; peptidase activity; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity