Active Recombinant Human WNT1 Inducible Signaling Pathway Protein 2

• Cat.No.: WISP2-644H
• Product Overview: Recombinant human WISP2 was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: CTGFL/WISP-2 is a 24.3 kDa protein that belongs to the CCN family of cysteine rich regulatory proteins. Members of this family stimulate mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types. The protein is expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart. In addition to promoting adhesion of osteoblasts, CTGFL/WISP-2 inhibits osteocalcin production, as well as binding of fibrinogen to integrin receptors
• Form: Supplied lyophilized with no additives. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not raise to neutral pH.
• Purity: >95% pure by SDS PAGE and HPLC
• Sequence: Region of CTGFL protein corresponding to amino acids MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCST
• Molecular Mass: 24.3 kDa
• Applications: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Bioactivity: The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.
• Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: WISP2 WNT1 inducible signaling pathway protein 2 [ Homo sapiens ]
• Official Symbol: WISP2
• Synonyms: WISP2; WNT1 inducible signaling pathway protein 2; CCN5; CT58; CTGF-L; OTTHUMP00000063227; wnt-1 signaling pathway protein 2; connective tissue growth factor-like protein; WNT1-inducible-signaling pathway protein 2; WISP-2; Connective tissue growth factor-related protein 58; OTTHUMP00000031770
• Gene ID: 8839
• mRNA Refseq: NM_003881
• Protein Refseq: NP_003872
• MIM: 603399
• UniProt ID: O76076
• Chromosome Location: 20q13.12
• Function: insulin-like growth factor binding; protein binding