Active Recombinant Mouse BACE2 Protein, His-Tagged

• Cat.No.: BACE2-33M
• Product Overview: Recombinant mouse BACE2 (Q9JL18) (Met1-Pro462) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-462 a.a.
• Description: BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major β-secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome(DS). Overexpression of BACE2 by lentivirus markedly reduced amyloid β protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.
• Form: Sterile PBS, pH 7.4
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2.
  The specific activity is >50 pmoles/min/μg.
• Molecular Mass: The recombinant mouse BACE2 comprises 454 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Bace2 beta-site APP-cleaving enzyme 2 [ Mus musculus (house mouse) ]
• Official Symbol: Bace2
• Synonyms: ARP1; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
• Gene ID: 56175
• mRNA Refseq: NM_019517.5
• Protein Refseq: NP_062390.3
• UniProt ID: Q9JL18