Species : |
Mouse |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
20-462 aa |
Description : |
BACE-2, also known as beta secretase 2, belongs to the peptidase A1 family. It is an aspartic protease that is a close homolog of BACE-1. However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in Aβ peptide production. It has been shown to play a key role in insulin receptor trafficking in the pancreas where it is expressed in β cells and affects glucose tolerance and was suggested as a promising target for improving β cell function in diabetes. |
Form : |
Liquid |
Bio-activity : |
Specific activity is > 20 pmol/min/μg in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to Mca- Pro-Leu-OH per minute at pH 3.5 at 25 centigrade. |
Molecular Mass : |
48.6 kDa (449 aa) |
AA Sequence : |
AVPALAPAPFTLPLQVARATNHRASAVPGLGTPELPRADGLALALEPVRATANFLAMVDNLQGDSGRGYYLEMLIGTPPQKVQILVDTGSSNFAVAGAPHSYIDTYFDSESSSTYHSKGFDVTVKYTQGSWTGFVGEDLVTIPKGFNSSFLVNIATIFESENFFLPGIKWNGILGLAYAALAKPSSSLETFFDSLVAQAKIPDIFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIGGQNLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARTSLIPEFSDGFWTGAQLACWTNSETPWAYFPKISIYLRDENASRSFRITILPQLYIQPMMGAGFNYECYRFGISSSTNALVIGATVMEGFYVVFDRAQRRVGFAVSPCAEIEGTTVSEISGPFSTEDIASNCVPAQALNEP |
Endotoxin : |
< 1 EU/μg of protein (determined by LAL method) |
Purity : |
> 95% by SDS-PAGE |
Applications : |
SDS-PAGE, Enzyme Activity |
Notes : |
For research use only. This product is not intended or approved for human, diagnostics or veterinary use. |
Storage : |
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : |
0.25 mg/mL (determined by Absorbance at 280 nm) |
Storage Buffer : |
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |