Active Recombinant Mouse Bace2 Protein (20-462aa), C-His-tagged

• Cat.No.: Bace2-11M
• Product Overview: Recombinant mouse BACE-2 (20-462aa), fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 20-462 aa
• Description: BACE-2, also known as beta secretase 2, belongs to the peptidase A1 family. It is an aspartic protease that is a close homolog of BACE-1. However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in Aβ peptide production. It has been shown to play a key role in insulin receptor trafficking in the pancreas where it is expressed in β cells and affects glucose tolerance and was suggested as a promising target for improving β cell function in diabetes.
• Form: Liquid
• Bio-activity: Specific activity is > 20 pmol/min/μg in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to Mca- Pro-Leu-OH per minute at pH 3.5 at 25 centigrade.
• Molecular Mass: 48.6 kDa (449 aa)
• AA Sequence: AVPALAPAPFTLPLQVARATNHRASAVPGLGTPELPRADGLALALEPVRATANFLAMVDNLQGDSGRGYYLEMLIGTPPQKVQILVDTGSSNFAVAGAPHSYIDTYFDSESSSTYHSKGFDVTVKYTQGSWTGFVGEDLVTIPKGFNSSFLVNIATIFESENFFLPGIKWNGILGLAYAALAKPSSSLETFFDSLVAQAKIPDIFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIGGQNLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARTSLIPEFSDGFWTGAQLACWTNSETPWAYFPKISIYLRDENASRSFRITILPQLYIQPMMGAGFNYECYRFGISSSTNALVIGATVMEGFYVVFDRAQRRVGFAVSPCAEIEGTTVSEISGPFSTEDIASNCVPAQALNEP
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280 nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Bace2 beta-site APP-cleaving enzyme 2 [ Mus musculus (house mouse) ]
• Official Symbol: Bace2
• Synonyms: BACE2; beta-site APP-cleaving enzyme 2; beta-secretase 2; ASP1; asp 1; memapsin-1; theta-secretase; aspartyl protease 1; beta-site APP cleaving enzyme 2; membrane-associated aspartic protease 1; beta-site amyloid precursor protein cleaving enzyme 2; ARP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
• Gene ID: 56175
• mRNA Refseq: NM_019517
• Protein Refseq: NP_062390
• UniProt ID: Q9JL18