Active Recombinant Mouse Fgf10 Protein (148 aa)

Cat.No. : Fgf10-420F
Product Overview : Recombinant mouse Fibroblast Growth Factor-10 (rmFGF-10) produced in E. coli is a single non-glycosylated polypeptide chain containing 148 amino acids. A fully biologically active molecule, rmFGF-10 has a molecular mass of 17.0 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
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  • Gene Information
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Description : Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares homology with KGF, and both KGF and FGF-10 activate the receptor FGFR2-IIIb. However, unlike KGF, which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during multi-organ development via mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development and is regulated by Shh during early development.
Source : E. coli
Species : Mouse
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : ED50 < 10 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0 × 10^5 units/mg.
Molecular Mass : 17.0 kDa, observed by reducing SDS-PAGE.
Protein Length : 148
AA Sequence : SSAGRHVRSYNHLQGDVRWRRLFSF TKYFLTIEKNGKVSGTKNEDCPYSV LEITSVEIGVVAVKAINSNYYLAMN KKGKLYGSKEFNNDCKLKERIEENG YNTYASFNWQHNGRQMYVALNGKGA PRRGQKTRRKNTSAHFLPMTIQT
Endotoxin : < 0.2 EU/μg, determined by LAL method.
Purity : > 95% as analyzed by SDS-PAGE and HPLC.
Storage : Lyophilized recombinant mouse Fibroblast Growth Factor-10 (rmFGF-10) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rmFGF-10 remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
Storage Buffer : Lyophilized after extensive dialysis against PBS.
Reconstitution : Reconstituted in ddH2O at 100 μg/mL.
Gene Name : Fgf10 fibroblast growth factor 10 [ Mus musculus ]
Official Symbol : Fgf10
Synonyms : FGF10; fibroblast growth factor 10; keratinocyte growth factor 2; Fgf-10; BB213776;
Gene ID : 14165
mRNA Refseq : NM_008002
Protein Refseq : NP_032028
UniProt ID : O35565

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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