Active Recombinant Human FGF10 Protein (Leu40-Ser208), C-His tagged, Animal-free, Carrier-free

• Cat.No.: FGF10-102H
• Product Overview: Recombinant Human FGF10 Protein (Leu40-Ser208) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Fibroblast Growth Factors-10 (FGF-10) is a 23.4 kDa member of the fibroblast Growth Factors with 208 amino acid residues. FGF-10 is mainly secreted from endometrial stromal cells, fibroblasts, peritubular cells, leydig cells, muller glia cells. FGF-10 a multifunctional mesenchymal-epithelial signaling Growth Factors, can regulate embryonic development, cell proliferation and cell differentiation. It has been associated with cancer and human genetic disorders.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 8 ng/mL. The specific activity of recombinant human FGF-10 is > 1.2 × 10⁵ IU/mg.
• Molecular Mass: The protein has a calculated MW of 20.06 kDa.
  The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Leu40-Ser208
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: FGF10 fibroblast growth factor 10 [ Homo sapiens (human) ]
• Official Symbol: FGF10
• Synonyms: FGF10; fibroblast growth factor 10; FGF-10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
• Gene ID: 2255
• mRNA Refseq: NM_004465
• Protein Refseq: NP_004456
• MIM: 602115
• UniProt ID: O15520