| Description : |
Flt3L, also known as Fms-related tyrosine kinase 3 ligand and SL cytokine, is a single-pass type I membrane protein. It is expressed by stromal cells and T cells. Flt3L signals through tyrosine kinase receptor Flt3/Flk2 to stimulate the proliferation of early hematopoietic progenitor cells. It synergizes with other growth factors, such as GM-CSF, IL-3 and CSF, to promote the differentiation of both myeloid and lymphoid cells. Alternative splicing and proteolytic cleavage of membrane-bound Flt3L generates a soluble extracellular domain (ECD) isoform with full biological activity. |
| Source : |
CHO |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 10 ng/mL, measured in a cell proliferation assay using AML5 cells. |
| Molecular Mass : |
24-30 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
GTPDCYFSHSPISSNFKVKFRELTD HLLKDYPVTVAVNLQDEKHCKALWS LFLAQRWIEQLKTVAGSKMQTLLED VNTEIHFVTSCTFQPLPECLRFVQT NISHLLKDTCTQLLALKPCIGKACQ NFSRCLEVQCQPDSSTLLPPRSPIA LEATELPEPRPRHHHHHH |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE. |
| Storage : |
Lyophilized recombinant murine Flt3L, His remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, murine Flt3L, His should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
