Active Recombinant Mouse Il11 Protein

• Cat.No.: Il11-085M
• Product Overview: Purified recombinant protein of Mouse interleukin 11 (Il11) without tag was expressed in E.coli.

Specification

• Description: Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3.
• Source: E. coli
• Species: Mouse
• Bio-activity: ED50 was determined by the dose-dependent stimulation of the proliferation of murine T11 cells is less than or equal to 2.0 ng/ml, corresponding to a specific activity of > 5 x 10^5 units/mg.
• Molecular Mass: 19.1 kDa
• AA Sequence: MPGPPAGSPRVSSDPRADLDSAVLL TRSLLADTRQLAAQMRDKFPADGDH SLDSLPTLAMSAGTLGSLQLPGVLT RLRVDLMSYLRHVQWLRRAGGPSLK TLEPELGALQARLERLLRRLQLLMS RLALPQAAPDQPVIPLGPPASAWGS IRAAHAILGGLHLTLDWAVRGLLLL KTRL
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.

Gene Information

• Gene Name: Il11 interleukin 11 [ Mus musculus (house mouse) ]
• Official Symbol: Il11
• Synonyms: Il11 interleukin 11; IL-11; interleukin-11
• Gene ID: 16156
• mRNA Refseq: NM_008350
• Protein Refseq: NP_032376
• UniProt ID: P47873

Reviews

10/28/2019

Potent in bone regeneration models.

11/26/2017

Suitable for cell culture.

01/10/2017

High yield in expression.

Q&As

How are in vivo models, including transgenic mice and knockdown strategies, utilized to study IL11's effects on tissue regeneration and homeostasis?

Transgenic mice and knockdown models explore IL11's impact on tissue regeneration.

Could you explain the experimental approaches, such as site-directed mutagenesis, employed to identify key residues in IL11's interaction with IL11RA?

Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.

What techniques like chromatin immunoprecipitation and DNA methylation analysis contribute to deciphering IL11's regulatory mechanisms?

Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.

Can you elaborate on the role of proteomics in identifying IL11-interacting proteins and potential cross-talk with other signaling pathways?

Proteomics identifies IL11-interacting proteins and potential cross-talk partners.

How do cell-based assays and luciferase reporter assays help investigate IL11's downstream signaling pathways through JAK-STAT activation?

Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.

What role does RNA sequencing play in understanding IL11-induced gene expression changes in various cell types?

RNA sequencing uncovers IL11-induced gene expression changes across different cell types.

What structural insights have X-ray crystallography and nuclear magnetic resonance spectroscopy provided about the IL11-IL11RA complex?

X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.

How do advanced imaging techniques like confocal microscopy and super-resolution microscopy visualize IL11 localization and distribution in tissues?

Confocal and super-resolution microscopy visualize IL11 localization in tissues.

How are techniques like surface plasmon resonance used to study IL11's binding kinetics to its receptor IL11RA?

Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.

What insights have CRISPR screening approaches provided regarding genes and pathways influenced by IL11 signaling?

CRISPR screening unveils genes and pathways influenced by IL11 signaling.