Recombinant Rat Il11 protein, His-PDI-tagged

• Cat.No.: Il11-4212R
• Product Overview: Recombinant Rat Il11 protein(Q99MF5)(22-199aa), fused with N-terminal His and PDI tag, was expressed in Yeast.

Specification

• Source: Yeast
• Species: Rat
• Tag: N-His-PDI
• Protein length: 22-199aa
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 78.0 kDa
• AASequence: PGPPAGSPRVSSDPRADLDSAVLLT RSLLADTRQLAAQMRDKFPADGDHN LDSLPTLAMSAGTLGSLQLPGVLTR LRVDLMSYFRHVQWLRRAAGPSLKT LEPELGALQARLERLLRRLQLLMSR LALPQAAPDQPAVPLGPPASAWGSI RAAHAILGGLHLTLDWAVRGLLLLK TRL
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Gene Information

• Gene Name: Il11 interleukin 11 [ Rattus norvegicus ]
• Official Symbol: Il11
• Synonyms: IL11; interleukin 11; interleukin-11; Il-11
• Gene ID: 171040
• mRNA Refseq: NM_133519
• Protein Refseq: NP_598203

Reviews

10/28/2019

Potent in bone regeneration models.

11/26/2017

Suitable for cell culture.

01/10/2017

High yield in expression.

Q&As

How are in vivo models, including transgenic mice and knockdown strategies, utilized to study IL11's effects on tissue regeneration and homeostasis?

Transgenic mice and knockdown models explore IL11's impact on tissue regeneration.

Could you explain the experimental approaches, such as site-directed mutagenesis, employed to identify key residues in IL11's interaction with IL11RA?

Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.

What techniques like chromatin immunoprecipitation and DNA methylation analysis contribute to deciphering IL11's regulatory mechanisms?

Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.

Can you elaborate on the role of proteomics in identifying IL11-interacting proteins and potential cross-talk with other signaling pathways?

Proteomics identifies IL11-interacting proteins and potential cross-talk partners.

How do cell-based assays and luciferase reporter assays help investigate IL11's downstream signaling pathways through JAK-STAT activation?

Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.

What role does RNA sequencing play in understanding IL11-induced gene expression changes in various cell types?

RNA sequencing uncovers IL11-induced gene expression changes across different cell types.

What structural insights have X-ray crystallography and nuclear magnetic resonance spectroscopy provided about the IL11-IL11RA complex?

X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.

How do advanced imaging techniques like confocal microscopy and super-resolution microscopy visualize IL11 localization and distribution in tissues?

Confocal and super-resolution microscopy visualize IL11 localization in tissues.

How are techniques like surface plasmon resonance used to study IL11's binding kinetics to its receptor IL11RA?

Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.

What insights have CRISPR screening approaches provided regarding genes and pathways influenced by IL11 signaling?

CRISPR screening unveils genes and pathways influenced by IL11 signaling.