Active Recombinant Mouse Il17a Protein

• Cat.No.: Il17a-095M
• Product Overview: Purified recombinant protein of Mouse IL-17A, the region of Ala26-Ala158, from gene Accession# NM_010552 and mouse IL-17F, the region of Arg29-Ala161, from gene Accession# NM_145856 without tag was expressed in E.coli.

Specification

• Description: This gene encodes a pro-inflammatory cytokine that is a member of the interleukin-17 family. The encoded protein plays a central role in host defense against diverse pathogens. The encoded protein is produced by activated T-cells and certain cell types of innate immune system. The active protein functions as either a homodimer with other interleukin-17 family members and signals through the interleukin-17 receptor to induce inflammatory cytokine production. Aberrant expression of this gene is associated with autoinflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
• Source: E. coli
• Species: Mouse
• Bio-activity: The ED50 is 125 - 175 ng/ml, corresponding to a specific activity 0.57 - 0.8 x 10^4 units/mg, determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of IL-6.
• Molecular Mass: 30 kDa
• Endotoxin: Less than 0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Storage Buffer: 10 mM NaH2PO4, 0.15 M NaCl, pH7.2
• Protein length: Ala26-Ala158;Arg29-Ala161

Gene Information

• Gene Name: Il17a interleukin 17A [ Mus musculus (house mouse) ]
• Official Symbol: Il17a
• Synonyms: Il17a; interleukin 17A; Il17; Ctla8; IL-17; Ctla-8; IL-17A; interleukin-17A; cytotoxic T-lymphocyte-associated antigen 8
• Gene ID: 16171
• mRNA Refseq: NM_010552
• Protein Refseq: NP_034682
• UniProt ID: Q62386

Reviews

12/16/2018

Good for receptor studies.

03/05/2018

Effective in Th17 differentiation.

03/08/2017

High bioactivity in various tissue types.

Q&As

Could you explain the role of IL17A in autoimmune disease progression using animal models?

Animal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.

Can you detail the protocols for inducing IL17A expression in T cells in vitro?

Inducing IL17A expression in T cells involves culturing them with specific cytokines.

What strategies elucidate IL17A's impact on tissue-specific inflammation in human samples?

Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.

Can you elaborate on the use of neutralizing antibodies in blocking IL17A's effects?

Neutralizing antibodies block IL17A's effects in cell cultures or animal models.

How does one perform a multiplex cytokine assay to quantify IL17A along with other cytokines?

Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.

How is the IL17A receptor (IL17RA) signaling pathway activated and studied?

IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.

What are the methods to investigate the impact of IL17A on epithelial barrier integrity?

Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.

How is IL17A's contribution to neutrophil recruitment assessed in inflammatory models?

Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.

What techniques are used to analyze IL17A-producing cell populations within a tissue?

Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.

What experimental design is employed to understand IL17A's involvement in cancer development?

Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.