Recombinant Human IL17A cell lysate

• Cat.No.: IL17A-001HCL
• Product Overview: Human IL17 / IL17A derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: IL17A interleukin 17A [ Homo sapiens ]
• Official Symbol: IL17A
• Synonyms: IL17A; interleukin 17A; CTLA8, IL17, interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8); interleukin-17A; cytotoxic T lymphocyte associated protein 8; IL 17; IL 17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); IL17; CTLA8; IL-17; IL-17A
• Gene ID: 3605
• mRNA Refseq: NM_002190
• Protein Refseq: NP_002181
• MIM: 603149
• UniProt ID: Q16552
• Chromosome Location: 6p12
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL23-mediated signaling events, organism-specific biosystem; IL27-mediated signaling events, organism-specific biosystem; Rheumatoid arthritis, organism-specific biosystem; Rheumatoid arthritis, conserved biosystem
• Function: cytokine activity

Reviews

12/16/2018

Good for receptor studies.

03/05/2018

Effective in Th17 differentiation.

03/08/2017

High bioactivity in various tissue types.

Q&As

Could you explain the role of IL17A in autoimmune disease progression using animal models?

Animal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.

Can you detail the protocols for inducing IL17A expression in T cells in vitro?

Inducing IL17A expression in T cells involves culturing them with specific cytokines.

What strategies elucidate IL17A's impact on tissue-specific inflammation in human samples?

Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.

Can you elaborate on the use of neutralizing antibodies in blocking IL17A's effects?

Neutralizing antibodies block IL17A's effects in cell cultures or animal models.

How does one perform a multiplex cytokine assay to quantify IL17A along with other cytokines?

Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.

How is the IL17A receptor (IL17RA) signaling pathway activated and studied?

IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.

What are the methods to investigate the impact of IL17A on epithelial barrier integrity?

Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.

How is IL17A's contribution to neutrophil recruitment assessed in inflammatory models?

Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.

What techniques are used to analyze IL17A-producing cell populations within a tissue?

Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.

What experimental design is employed to understand IL17A's involvement in cancer development?

Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.