Active Recombinant Mouse Il1a Protein (156 aa)
|Product Overview :||Recombinant Mouse Il1a Protein without tag was expressed in E. coli.|
- Gene Information
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|Description :||IL-1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.|
|Source :||E. coli|
|Form :||Sterile Filtered White lyophilized (freeze-dried) powder.|
|Bio-activity :||Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.002 ng/mL, corresponding to a specific activity of > 5.0 × 10^8 units/mg.|
|Molecular Mass :||Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.|
|Protein Length :||156|
|AA Sequence :||SAPYTYQSDLRYKLMKLVRQKFVMN DSLNQTIYQDVDKHYLSTTWLNDLQ QEVKFDMYAYSSGGDDSKYPVTLKI SDSQLFVSAQGEDQPVLLKELPETP KLITGSETDLIFFWKSINSKNYFTS AAYPELFIATKEQSRVHLARGLPSM TDFQIS|
|Endotoxin :||Less than 1 EU/mg of rMuIL-1 alpha as determined by LAL method.|
|Purity :||>97% by SDS-PAGE and HPLC analyses|
|Storage :||This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.|
|Storage Buffer :||Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.|
|Reconstitution :||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.|
|Gene Name :||Il1a interleukin 1 alpha [ Mus musculus ]|
|Official Symbol :||Il1a|
|Synonyms :||IL1A; interleukin 1 alpha; interleukin-1 alpha; IL-1 alpha; Il-1a;|
|Gene ID :||16175|
|mRNA Refseq :||NM_010554|
|Protein Refseq :||NP_034684|
|UniProt ID :||P01582|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
Customer Reviews (3)Write a review
High solubility. -
Good for intracellular staining. -
Effective in apoptosis assays. -
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