Active Recombinant Mouse Il1a Protein
Cat.No. : | Il1a-098M |
Product Overview : | Recombinant protein of mouse interleukin-1a/IL-1a (Ser115-Ser270) without tag was expressed in E.coli. |
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Description : | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
Source : | E. coli |
Species : | Mouse |
Bio-activity : | ED50 is less than 0.01 ng/ml as determined by the dose-dependent stimulation of mouse D10S cells. Specific Activity of 1.0 x 108 IU/mg. |
Molecular Mass : | 18 kDa |
Endotoxin : | < 0.1 EU per µg protein as determined by LAL test |
Purity : | >95%, as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for at least 6 months from date of receipt under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. |
Storage Buffer : | Lyophilized from a 0.2 µM filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 8.0. |
Reconstitution : | Resuspend the protein in the desired concentration in proper buffer. |
Protein length : | Ser115-Ser270 |
Gene Name : | Il1a interleukin 1 alpha [ Mus musculus (house mouse) ] |
Official Symbol : | Il1a |
Synonyms : | Il1a; interleukin 1 alpha; Il-1a; interleukin-1 alpha; IL-1 alpha |
Gene ID : | 16175 |
mRNA Refseq : | NM_010554 |
Protein Refseq : | NP_034684 |
UniProt ID : | P01582 |
Products Types
◆ Lysates | ||
IL1A-2911HCL | Recombinant Human IL1A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewHigh solubility.
Good for intracellular staining.
Effective in apoptosis assays.
Q&As (10)
Ask a questionCross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
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