Active Recombinant Mouse Il1a Protein

Cat.No. : Il1a-098M
Product Overview : Recombinant protein of mouse interleukin-1a/IL-1a (Ser115-Ser270) without tag was expressed in E.coli.
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Description : Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Source : E. coli
Species : Mouse
Bio-activity : ED50 is less than 0.01 ng/ml as determined by the dose-dependent stimulation of mouse D10S cells.
Specific Activity of 1.0 x 108 IU/mg.
Molecular Mass : 18 kDa
Endotoxin : < 0.1 EU per µg protein as determined by LAL test
Purity : >95%, as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Storage : Store at -80 centigrade.
Storage Buffer : Lyophilized from a 0.2 µM filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 8.0.
Reconstitution : Resuspend the protein in the desired concentration in proper buffer.
Protein length : Ser115-Ser270
Gene Name : Il1a interleukin 1 alpha [ Mus musculus (house mouse) ]
Official Symbol : Il1a
Synonyms : Il1a; interleukin 1 alpha; Il-1a; interleukin-1 alpha; IL-1 alpha
Gene ID : 16175
mRNA Refseq : NM_010554
Protein Refseq : NP_034684
UniProt ID : P01582

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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Reviews
04/08/2021

    High solubility.

    07/02/2020

      Good for intracellular staining.

      03/13/2019

        Effective in apoptosis assays.

        Q&As (10)

        Ask a question
        What methods are employed to dissect the intricate cross-talk between IL-1α and other cytokines, shedding light on its role in immune modulation? 11/02/2021

        Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.

        What experimental approaches allow researchers to dissect the paradoxical roles of IL-1α as both a pro-inflammatory and tissue repair mediator in distinct contexts? 09/22/2021

        IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.

        How are cutting-edge techniques like single-cell RNA sequencing utilized to unravel the heterogeneity in IL-1α-responsive cell populations and their functional implications? 06/07/2021

        Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.

        How is the intricate web of IL-1α's pro-inflammatory signaling pathways decoded using advanced experimental techniques? 05/26/2021

        IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.

        How are state-of-the-art genetic editing tools such as CRISPR-Cas9 applied to unravel IL-1α's precise contributions to inflammatory diseases? 04/06/2021

        Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.

        How do researchers manipulate in vitro co-culture systems to dissect the dynamic interplay between immune cells and stromal cells in IL-1α-mediated responses? 06/18/2020

        In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.

        What experimental strategies enable the identification of specific cellular receptors and downstream effectors responsible for IL-1α's diverse physiological effects? 03/19/2020

        Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.

        How are sophisticated imaging techniques like live-cell microscopy harnessed to capture the real-time dynamics of IL-1α release and its impact on neighboring cells? 12/02/2019

        Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.

        How is the quantitative relationship between IL-1α levels and disease progression established through longitudinal studies and statistical modeling? 07/15/2018

        Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.

        How do multi-omics analyses, including transcriptomics and proteomics, provide a holistic view of the network of molecules influenced by IL-1α signaling? 06/04/2018

        Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.

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