Recombinant Guinea pig Il1a protein, His & S-tagged
|Product Overview :||Recombinant Guinea pig Il1a aa. (Ser111~Glu204 (Accession # Q60480)) fused with N-terminal His & S tag was produced in E. coli cells.|
- Gene Information
- Related Products
|Source :||E. coli|
|Species :||Guinea pig|
|Tag :||His & S|
|Form :||Freeze-dried powder|
|Molecular Mass :||Predicted Molecular Mass: 16.5kDa|
|Protein length :||Ser111~Glu204 (Accession # Q60480)|
|Endotoxin :||<1.0EU per 1µg (determined by the LAL method)|
|Characteristic :||The isoelectric point is 7.9.|
|Applications :||SDS-PAGE; WB; ELISA; IP.|
|Stability :||The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.|
|Storage :||Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.|
|Storage buffer :||Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.|
|Reconstitution :||Reconstitute in sterile PBS, pH7.2-pH7.4.|
|Gene Name :||Il1a interleukin 1 alpha [ Cavia porcellus (domestic guinea pig) ]|
|Official Symbol :||Il1a|
|Synonyms :||Il1a; interleukin-1 alpha|
|Gene ID :||100135555|
|mRNA Refseq :||XM_003465056.2|
|Protein Refseq :||XP_003465104.1|
|UniProt ID :||Q60480|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
Customer Reviews (3)Write a review
High solubility. -
Good for intracellular staining. -
Effective in apoptosis assays. -
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