Active Recombinant Mouse Interleukin 12b
|Product Overview :||RecombinantMouse Il12b protein was expressed in Spodopterafrugiperda. Met23-Ser335(p40) & Arg23-Ala215 (p35).|
- Gene Information
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|Cat. No. :||Il12b-644M|
|Description :||Il12b is a heterodimericpleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35)subunit. Il12b isproduced by macrophages and B lymphocytes and has been shown to have multipleeffects on T cells and Natural Killer (NK) cells. Some of these Il12b activitiesinclude the induction of IFNγ and TNF in resting and activated T and NK cells,the enhancement of cytotoxic activity of resting NK and T cells, the stimulationof resting T cell proliferation in the presence of a comitogen, and theenhancement of NK cell proliferation.|
|Source :||Spodopterafrugiperda,Sf 21 (baculovirus)-derived.|
|Form :||Lyophilizedfrom a 0.2 um filtered solution in PBS with BSA as a carrier protein.|
|N-terminalSequence :||Met23 (p40) &Arg23 (p35)|
|Activity :||Measuredin a cell proliferation assay using PHAactivated mouse splenocytes. The ED50for this effect is typically 0.01-0.1 ng/mL.|
|Endotoxin Level :||<1.0EU per 1μg of the protein by the LAL method.|
|SDS-PAGE :||70 kDa,non-reducing conditions.|
|Purity :||>97%,by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Reconstitution :||Reconstituteat 100μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.|
|Storage :||Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.|
|Gene Name :||Il12b interleukin 12b [ Musmusculus ]|
|Official Symbol :||Il12b|
|Synonyms :||Il12b; interleukin12b; p40; Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40;IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor40 kDa subunit|
|Gene ID :||16160|
|mRNA Refseq :||NM_008352|
|Protein Refseq :||NP_032378|
|UniProt ID :||P43432|
|Chromosome Location :||11|
|Pathway :||Africantrypanosomiasis; Allograft rejection; Amoebiasis; Cytokine-cytokine receptorinteraction; Herpes simplex infection; Influenza A; Jak-STAT signalingpathway; Legionellosis; Leishmaniasis; Measles; Pertussis; RIG-I-likereceptor signaling pathway; Toll-like receptor signaling pathway; Toxoplasmosis;Type I diabetes mellitus|
|Function :||contributes_tocytokine activity; cytokine receptor activity; contributes_to cytokinereceptor binding; identical protein binding; interleukin-12 alpha subunit binding;protein binding; protein heterodimerization activity|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
ChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
Customer Reviews (3)Write a review
Good for cytokine assays. -
Effective in Th2 cell differentiation. -
High yield in expression. -
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