Active Recombinant Mouse Interleukin 12b

• Cat.No.: Il12b-644M
• Product Overview: RecombinantMouse Il12b protein was expressed in Spodopterafrugiperda. Met23-Ser335(p40) & Arg23-Ala215 (p35).

Specification

• Cat. No.: Il12b-644M
• Description: Il12b is a heterodimericpleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35)subunit. Il12b isproduced by macrophages and B lymphocytes and has been shown to have multipleeffects on T cells and Natural Killer (NK) cells. Some of these Il12b activitiesinclude the induction of IFNγ and TNF in resting and activated T and NK cells,the enhancement of cytotoxic activity of resting NK and T cells, the stimulationof resting T cell proliferation in the presence of a comitogen, and theenhancement of NK cell proliferation.
• Source: Spodopterafrugiperda,Sf 21 (baculovirus)-derived.
• Form: Lyophilizedfrom a 0.2 um filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Met23 (p40) &Arg23 (p35)
• Activity: Measuredin a cell proliferation assay using PHAactivated mouse splenocytes. The ED50for this effect is typically 0.01-0.1 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 70 kDa,non-reducing conditions.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Il12b interleukin 12b [ Musmusculus ]
• Official Symbol: Il12b
• Synonyms: Il12b; interleukin12b; p40; Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40;IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor40 kDa subunit
• Gene ID: 16160
• mRNA Refseq: NM_008352
• Protein Refseq: NP_032378
• UniProt ID: P43432
• Chromosome Location: 11
• Pathway: Africantrypanosomiasis; Allograft rejection; Amoebiasis; Cytokine-cytokine receptorinteraction; Herpes simplex infection; Influenza A; Jak-STAT signalingpathway; Legionellosis; Leishmaniasis; Measles; Pertussis; RIG-I-likereceptor signaling pathway; Toll-like receptor signaling pathway; Toxoplasmosis;Type I diabetes mellitus
• Function: contributes_tocytokine activity; cytokine receptor activity; contributes_to cytokinereceptor binding; identical protein binding; interleukin-12 alpha subunit binding;protein binding; protein heterodimerization activity