Active Recombinant Mouse Interleukin 12b

Cat.No. : Il12b-644M
Product Overview : RecombinantMouse Il12b protein was expressed in Spodopterafrugiperda. Met23-Ser335(p40) & Arg23-Ala215 (p35).
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Cat. No. : Il12b-644M
Description : Il12b is a heterodimericpleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35)subunit. Il12b isproduced by macrophages and B lymphocytes and has been shown to have multipleeffects on T cells and Natural Killer (NK) cells. Some of these Il12b activitiesinclude the induction of IFNγ and TNF in resting and activated T and NK cells,the enhancement of cytotoxic activity of resting NK and T cells, the stimulationof resting T cell proliferation in the presence of a comitogen, and theenhancement of NK cell proliferation.
Source : Spodopterafrugiperda,Sf 21 (baculovirus)-derived.
Form : Lyophilizedfrom a 0.2 um filtered solution in PBS with BSA as a carrier protein.
N-terminalSequence : Met23 (p40) &Arg23 (p35)
Activity : Measuredin a cell proliferation assay using PHAactivated mouse splenocytes. The ED50for this effect is typically 0.01-0.1 ng/mL.
Endotoxin Level : <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE : 70 kDa,non-reducing conditions.
Purity : >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstituteat 100μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
Storage : Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
Tag : Non
Gene Name : Il12b interleukin 12b [ Musmusculus ]
Official Symbol : Il12b
Synonyms : Il12b; interleukin12b; p40; Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40;IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor40 kDa subunit
Gene ID : 16160
mRNA Refseq : NM_008352
Protein Refseq : NP_032378
UniProt ID : P43432
Chromosome Location : 11
Pathway : Africantrypanosomiasis; Allograft rejection; Amoebiasis; Cytokine-cytokine receptorinteraction; Herpes simplex infection; Influenza A; Jak-STAT signalingpathway; Legionellosis; Leishmaniasis; Measles; Pertussis; RIG-I-likereceptor signaling pathway; Toll-like receptor signaling pathway; Toxoplasmosis;Type I diabetes mellitus
Function : contributes_tocytokine activity; cytokine receptor activity; contributes_to cytokinereceptor binding; identical protein binding; interleukin-12 alpha subunit binding;protein binding; protein heterodimerization activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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Customer Reviews (3)

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    Good for cytokine assays.


      Effective in Th2 cell differentiation.


        High yield in expression.

        Q&As (10)

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        Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation? 05/12/2021

        ChIP-seq identifies regulatory elements controlling IL12B gene expression.

        Can you elaborate on the use of reporter assays to monitor IL12B promoter activity? 05/02/2021

        Reporter assays monitor IL12B promoter activity under varying conditions.

        What methods evaluate IL12B's response to different pathogenic stimuli in primary cells? 10/30/2020

        Primary cell stimulation assays gauge IL12B response to pathogens.

        How does siRNA knockdown of IL12B elucidate its role in immune cell activation? 05/25/2020

        siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

        How does recombinant protein expression and purification aid in IL12B functional studies? 04/30/2020

        Recombinant protein expression enables functional IL12B studies in vitro.

        How does co-immunoprecipitation validate IL12B's association with signaling partners? 04/12/2018

        Co-IP validates IL12B's binding to specific signaling partners.

        What techniques are employed to analyze the impact of IL12B genetic variants on protein function? 12/03/2017

        Genetic variant analysis reveals impacts on IL12B protein function.

        How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets? 10/31/2017

        Flow cytometry profiles IL12B expression across diverse immune cell types.

        What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation? 09/29/2017

        Cytokine secretion assays measure IL12B's response kinetics to stimuli.

        What are the methods to assess IL12B's protein-protein interactions in a cellular context? 08/22/2017

        Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.

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