Recombinant Human Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Cat.No. : | IL12B-670H |
Product Overview : | Recombinant HumanIL12B is a glycosylated heterodimeric polypeptide containing 503 amino acids. |
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Cat. No. : | IL12B-670H |
Description : | This gene encodes asubunit of interleukin 12, a cytokine that acts on T and natural killercells, and has a broad array of biological activities. Interleukin 12 is adisulfide-linked heterodimer composed of the 40 kD cytokine receptor likesubunit encoded by this gene, and a 35 kD subunit encoded by IL12A. |
Source : | CHO cells |
Species : | Human |
Form : | Lyophilized from asterile filtered solution containing PBS, pH 7.4. |
Purity : | >98% by HPLC andSDS-PAGE. |
Molecular Weight : | 75 kDa; comprised ofdisulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. |
Endotoxin Level : | <0.1 ng/μg ofRecombinant Human IL12. |
Biological Activity : | Determined by itsability to stimulate the proliferation of PHA-activated human T lymphoblasts.The ED50 for this effect is 0.1-0.2 ng/mL. |
Amino Acid Sequence : | p35 Subunit:RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTKNESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAVIDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS; p40 Subunit:IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHKGGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGSSDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDIIKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRKNASISVRAQD RYYSSSWSEW ASVPCS |
Reconstitution : | Centrifuge vialprior to opening. Add sterile distilled water or aqueous buffer containing0.1% BSA to a concentration of 0.1-1.0 mg/mL. Please note that the additionof any carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Further dilutions should bemade in appropriate buffered solutions. |
Storage : | Stable at 2-8°C, butbest kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeatedfreeze/thaw cycles. |
OfficialSymbol : | IL12B |
Pathways : | Africantrypanosomiasis; Allograft rejection; Amoebiasis; Chagas disease (Americantrypanosomiasis); Cytokine-cytokine receptor interaction; Cytokines andInflammatory Response; Herpes simplex infection; IL12-mediated signalingevents; IL23-mediated signaling events; IL27-mediated signaling events;Influenza A; Jak-STAT signaling pathway; Legionellosis; Leishmaniasis;Measles |
Tag : | Non |
Gene Name : | IL12B interleukin 12B (naturalkiller cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2,p40) [ Homo sapiens ] |
Synonyms : | IL12B; Interleukin12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); CLMF; NKSF; CLMF2; NKSF2; IL-12B; interleukin-12subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40;OTTHUMP00000160820; interleukin 12, p40; interleukin-12 beta chain; NK cellstimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDasubunit; natural killer cell stimulatory factor, 40 kD subunit; Interleukin-12 subunit beta; Cytotoxic lymphocyte maturation factor 40 kDa subunit |
Gene ID : | 3593 |
mRNA Refseq : | NM_002187 |
Protein Refseq : | NP_002178 |
MIM : | 161561 |
UniProt ID : | P29460 |
Chromosome Location : | 5q31.1-q33.1 |
Function : | Contributes tocytokine activity; cytokine receptor activity; contributes to growthfactor activity; identical protein binding; interleukin-12 alpha subunitbinding; interleukin-12 receptor binding; contributes to interleukin-12receptor binding; contributes to interleukin-23 receptor binding; proteinbinding; protein heterodimerization activity; signal transducer activity |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
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