Recombinant Human Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

• Cat.No.: IL12B-670H
• Product Overview: Recombinant HumanIL12B is a glycosylated heterodimeric polypeptide containing 503 amino acids.

Specification

• Cat. No.: IL12B-670H
• Description: This gene encodes asubunit of interleukin 12, a cytokine that acts on T and natural killercells, and has a broad array of biological activities. Interleukin 12 is adisulfide-linked heterodimer composed of the 40 kD cytokine receptor likesubunit encoded by this gene, and a 35 kD subunit encoded by IL12A.
• Source: CHO cells
• Species: Human
• Form: Lyophilized from asterile filtered solution containing PBS, pH 7.4.
• Purity: >98% by HPLC andSDS-PAGE.
• Molecular Weight: 75 kDa; comprised ofdisulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
• Endotoxin Level: <0.1 ng/μg ofRecombinant Human IL12.
• Biological Activity: Determined by itsability to stimulate the proliferation of PHA-activated human T lymphoblasts.The ED50 for this effect is 0.1-0.2 ng/mL.
• Amino Acid Sequence: p35 Subunit:RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTKNESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAVIDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS; p40 Subunit:IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHKGGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGSSDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDIIKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRKNASISVRAQD RYYSSSWSEW ASVPCS
• Reconstitution: Centrifuge vialprior to opening. Add sterile distilled water or aqueous buffer containing0.1% BSA to a concentration of 0.1-1.0 mg/mL. Please note that the additionof any carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Further dilutions should bemade in appropriate buffered solutions.
• Storage: Stable at 2-8°C, butbest kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeatedfreeze/thaw cycles.
• OfficialSymbol: IL12B
• Pathways: Africantrypanosomiasis; Allograft rejection; Amoebiasis; Chagas disease (Americantrypanosomiasis); Cytokine-cytokine receptor interaction; Cytokines andInflammatory Response; Herpes simplex infection; IL12-mediated signalingevents; IL23-mediated signaling events; IL27-mediated signaling events;Influenza A; Jak-STAT signaling pathway; Legionellosis; Leishmaniasis;Measles

Gene Information

• Gene Name: IL12B interleukin 12B (naturalkiller cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2,p40) [ Homo sapiens ]
• Synonyms: IL12B; Interleukin12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); CLMF; NKSF; CLMF2; NKSF2; IL-12B; interleukin-12subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40;OTTHUMP00000160820; interleukin 12, p40; interleukin-12 beta chain; NK cellstimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDasubunit; natural killer cell stimulatory factor, 40 kD subunit; Interleukin-12 subunit beta; Cytotoxic lymphocyte maturation factor 40 kDa subunit
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• MIM: 161561
• UniProt ID: P29460
• Chromosome Location: 5q31.1-q33.1
• Function: Contributes tocytokine activity; cytokine receptor activity; contributes to growthfactor activity; identical protein binding; interleukin-12 alpha subunitbinding; interleukin-12 receptor binding; contributes to interleukin-12receptor binding; contributes to interleukin-23 receptor binding; proteinbinding; protein heterodimerization activity; signal transducer activity