Recombinant Golden hamster IL12B protein, His&Myc-tagged
Cat.No. : | IL12B-7476G |
Product Overview : | Recombinant Golden hamster IL12B protein(Q8CJE6)(23-327aa), fused with N-terminal His tag and C-terminal Myc tag, was expressed in E.coli. |
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Source : | E.coli |
Species : | Golden hamster |
Tag : | His&Myc |
Protein length : | 23-327a.a. |
Form : | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Molecular Mass : | 39.8 kDa |
AASequence : | IWELEKDVYVVEVDWSPDAAGERVV LTCDTSEEDDIIWTSDKNSEAVGSG KTLTIQVKEFSNAGQYTCHKGGKTL SHSRLLLHKKENGIWSTDILKDQKD PKNKTFLKCEAANYSGRFTCWWLTA ISTDLKFNVKSSSSSSDSRAVTCGA ASLSAEKVTVDRKDYQKYSVACQED ITCPTAEETLPIGLVMEAQHKYKYE NYSTGFFIRDIIKPDPPKNLQLKPL RGSQMELSWEYPDSWSTPHSYFSLK FHVQVHRKRERKDESQFVDKTSATI RCSKGAEVRVRAQDHYYNSSWSRWV SVPCS |
Purity : | Greater than 85% as determined by SDS-PAGE. |
Storage : | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
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