Active Recombinant Mouse Interleukin 12b, His-tagged
Cat.No. : | Il12b-5647M |
Product Overview : | RecombinantMouse Il12b protein was expressed in Murine myeloma cellline. Gln20-Glu561,with a C-terminal 6-His tag. |
- Specification
- Gene Information
- Related Products
- Download
Cat. No. : | Il12b-5647M |
Description : | Il12b isa 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF Rfamily ofcytokine receptors. Il12b is a common subunit of both the IL-12 andIL-23 receptor complexes which play distinct but related roles in T cell mediatedinflammatory reactions. Mature mouse Il12b contains a 546 amino acid (aa) extracellulardomain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmicdomain. Within the ECD, mouse Il12b shares 85% and 52% aa sequence identitywith rat and human Il12b, respectively. It shares 16%-21% aa sequenceidentity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 andIL-23 are disulfide linked heterodimeric cytokines that share a common p40subunit. |
Source : | Mammalian cells |
Form : | Lyophilized from a0.2 um filtered solution in PBS. |
N-terminalSequence : | Gln20 predicted |
MolecularWeight : | 61.3 kDa |
Activity : | Measuredby its binding ability in a functional ELISA. Immobilized rmIL-12 R 1 at 10 μg/mL(100 μL/well) can bind rmIL-12 with a linear range of 0.3-20 μg/mL. |
Endotoxin Level : | <1.0EU per 1μg of the protein by the LAL method. |
SDS-PAGE : | 95-110kDa, reducing conditions. |
Purity : | >90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : | 100 μg/mLin sterile PBS |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thawcycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution. |
Protein length : | Gln20-Glu561 |
Gene Name : | Il12b interleukin 12b [ Musmusculus ] |
Official Symbol : | Il12b |
Synonyms : | Il12b; interleukin12b; p40;Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40; IL-12 p40;IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor40 kDa subunit |
Gene ID : | 16160 |
mRNA Refseq : | NM_008352 |
Protein Refseq : | NP_032378 |
UniProt ID : | Q60837 |
Chromosome Location : | 11 |
Pathway : | Africantrypanosomiasis; Allograft rejection; Amoebiasis; Chagas disease (Americantrypanosomiasis); Cytokine-cytokine receptor interaction; Herpes simplexinfection; Influenza A; Jak-STAT signaling pathway; Legionellosis; Leishmaniasis;Measles; Pertussis; RIG-I-like receptor signaling pathway; Toll-like receptorsignaling pathway; Toxoplasmosis |
Function : | contributes_tocytokine activity; cytokine receptor activity; contributes_to cytokinereceptor binding; identical protein binding; interleukin-12 alpha subunitbinding; protein binding; protein heterodimerization activity |
Products Types
◆ Recombinant Protein | ||
IL12B-5F | Recombinant Feline IL12B Protein, His (Fc)-Avi-tagged | +Inquiry |
IL12B-503H | Recombinant Human IL12B Protein, Fc-tagged | +Inquiry |
IL12B-4330S | Recombinant Sheep IL12B Protein | +Inquiry |
IL12B-3433H | Recombinant Human IL12B protein, His-tagged | +Inquiry |
Il12b-4492M | Recombinant Mouse Il12b Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
IL12B-1000MCL | Recombinant Marmoset IL12B cell lysate | +Inquiry |
IL12B-2731HCL | Recombinant Human IL12B cell lysate | +Inquiry |
IL12B-1197RCL | Recombinant Rat IL12B cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Reviews
- Q&As
Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
Ask a Question for All Il12b Products
Required fields are marked with *
My Review for All Il12b Products
Required fields are marked with *
Inquiry Basket