Active Recombinant Mouse Interleukin 12b, His-tagged

• Cat.No.: Il12b-5647M
• Product Overview: RecombinantMouse Il12b protein was expressed in Murine myeloma cellline. Gln20-Glu561,with a C-terminal 6-His tag.

Specification

• Cat. No.: Il12b-5647M
• Description: Il12b isa 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF Rfamily ofcytokine receptors. Il12b is a common subunit of both the IL-12 andIL-23 receptor complexes which play distinct but related roles in T cell mediatedinflammatory reactions. Mature mouse Il12b contains a 546 amino acid (aa) extracellulardomain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmicdomain. Within the ECD, mouse Il12b shares 85% and 52% aa sequence identitywith rat and human Il12b, respectively. It shares 16%-21% aa sequenceidentity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 andIL-23 are disulfide linked heterodimeric cytokines that share a common p40subunit.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: Gln20 predicted
• MolecularWeight: 61.3 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rmIL-12 R 1 at 10 μg/mL(100 μL/well) can bind rmIL-12 with a linear range of 0.3-20 μg/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 95-110kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use a manual defrost freezer and avoid repeated freeze-thawcycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
• Protein length: Gln20-Glu561

Gene Information

• Gene Name: Il12b interleukin 12b [ Musmusculus ]
• Official Symbol: Il12b
• Synonyms: Il12b; interleukin12b; p40;Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40; IL-12 p40;IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor40 kDa subunit
• Gene ID: 16160
• mRNA Refseq: NM_008352
• Protein Refseq: NP_032378
• UniProt ID: Q60837
• Chromosome Location: 11
• Pathway: Africantrypanosomiasis; Allograft rejection; Amoebiasis; Chagas disease (Americantrypanosomiasis); Cytokine-cytokine receptor interaction; Herpes simplexinfection; Influenza A; Jak-STAT signaling pathway; Legionellosis; Leishmaniasis;Measles; Pertussis; RIG-I-like receptor signaling pathway; Toll-like receptorsignaling pathway; Toxoplasmosis
• Function: contributes_tocytokine activity; cytokine receptor activity; contributes_to cytokinereceptor binding; identical protein binding; interleukin-12 alpha subunitbinding; protein binding; protein heterodimerization activity

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.