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Active Recombinant Mouse Interleukin 18

Cat.No. : II18-272M
Product Overview : Recombinant mouse interleukin 1, beta was expressed inE.coli,encoding the matured Mouse IL-18 protein sequence (corresponding to 36-192 aa).
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Cat. No. : II18-272M
Description : Interleukin 18 (IL-18) is an 18-kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
Source : Escherichia Coli.
Purity : Greater than 90% purity as confirmed on SDS-PAGE by Coomassie brilliant blue staining.
Activity : Induction of IFN-γ by mouse IL-18 receptor transfected KG-1 cell [human myelomonocyte; ATCC CCL246] in response to the recombinant mouse IL-18 was measured using human IFN-γ ELISA.
Formulation : Lyophilized in PBS containing 1% sucrose. Reconstitute in 2 mL of ice-cold distilled water on ice.
Storage : Lyophilized Mouse IL-18 is stable for 24 months from the date of manufacture when store at -20℃ or below. After reconstitution, avoid repeated freezing and thawing. The IL-18 can be stored for 1 week at 4℃. For storage, prepare appropriate aliquots and freeze them at -80℃.
Gene Name : Il18 interleukin 18 [ Mus musculus ]
Synonyms : Il18; interleukin 18; Igif; Il-18; Il18; interleukin 18; IL-1 gamma; interleukin-18; interleukin-1 gamma; IFN-gamma-inducing factor; interferon-gamma-inducing factor
Gene ID : 16173
mRNA Refseq : NM_008360
Protein Refseq : NP_032386
UniProt ID : P70380
Chromosome Location : 9F;9 29.0 cM
Pathway : Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; NOD-like receptor signaling
Function : cytokine activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.



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