Active Recombinant Human IL18 protein, Fc-tagged

• Cat.No.: IL18 -1575H
• Product Overview: Recombinant Human IL18 protein(Phe20-Arg356), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5.
• Bio-activity: Biotinylated Human IL-18 R1, His,Avitag captured on Biotin CAP-Series S Sensor Chip can bind Human IL-18, Tag Free (Premixed with Human IL-18 R beta / IL-1 R7, Fc Tag) with an affinity constant of 5.87 nM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 64.9 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe20-Arg356
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
• Official Symbol: IL18
• Synonyms: IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g; MGC12320
• Gene ID: 3606
• mRNA Refseq: NM_001243211
• Protein Refseq: NP_001230140
• MIM: 600953
• UniProt ID: Q14116